FN Archimer Export Format
PT J
TI Non-lethal sampling for assessment of mitochondrial function does not affect metabolic rate and swimming performance
BT 
AF Thoral, Elisa
   Dargère, Lauréliane
   Medina-Suárez, Ione
   Clair, Angéline
   Averty, Laetitia
   Sigaud, Justine
   Morales, Anne
   Salin, Karine
   Teulier, Loïc
AS 1:1,2;2:1;3:3;4:4;5:4;6:4;7:1;8:5;9:1;
FF 1:;2:;3:;4:;5:;6:;7:;8:PDG-RBE-PHYTNESS;9:;
C1 Univ Lyon, Université Claude Bernard Lyon 1, CNRS, ENTPE, UMR 5023 LEHNA, F-69622, Villeurbanne, France
   Department of Biology, Section for Evolutionary Ecology, Lund University, Sölvegatan 37, Lund 223 62, Sweden
   Instituto de Oceanografía y Cambio Global, IOCAG, Universidad de Las Palmas de Gran Canaria, Unidad Asociada ULPGC-CSIC, Campus de Taliarte, 35214 Telde, Gran Canaria, Canary Islands, Spain
   Plateforme Animalerie Conventionnelle et Sauvage Expérimentale de la Doua (ACSED), Fédération de Recherche 3728, Univ Lyon, Université Claude Bernard Lyon 1, CNRS, ENS-Lyon, INRAE, INSA, VetAgroSup 69622, Villeurbanne, France
   Ifremer, CNRS, RD, Laboratory of Environmental Marine Sciences, Université de Brest, 29280 Plouzané, France
C2 UNIV LYON, FRANCE
   UNIV LUND, SWEDEN
   UNIV LAS PALMAS GRAN CANARIA, SPAIN
   UNIV LYON, FRANCE
   IFREMER, FRANCE
SI BREST
SE PDG-RBE-PHYTNESS
UM LEMAR
IN WOS Ifremer UMR
   copubli-france
   copubli-europe
   copubli-univ-france
IF 6.3
TC 2
UR https://archimer.ifremer.fr/doc/00870/98205/107595.pdf
LA English
DT Article
DE ;red muscle;whole-animal performance;repeatability;goldfish
AB A fundamental issue in the metabolic field is whether it is possible to understand underlying mechanisms that characterize individual variation. Whole-animal performance relies on mitochondrial function as it produces energy for cellular processes. However, our lack of longitudinal measures to evaluate how mitochondrial function can change within and among individuals and with environmental context makes it difficult to assess individual variation in mitochondrial traits. The aims of this study were to test the repeatability of muscle mitochondrial metabolism by performing two biopsies of red muscle, and to evaluate the effects of biopsies on whole-animal performance in goldfish Carassius auratus . Our results show that basal mitochondrial respiration and net phosphorylation efficiency are repeatable at 14-day intervals. We also show that swimming performance (optimal cost of transport and critical swimming speed) was repeatable in biopsied fish, whereas the repeatability of individual oxygen consumption (standard and maximal metabolic rates) seemed unstable over time. However, we noted that the means of individual and mitochondrial traits did not change over time in biopsied fish. This study shows that muscle biopsies allow the measurement of mitochondrial metabolism without sacrificing animals and that two muscle biopsies 14 days apart affect the intraspecific variation in fish performance without affecting average performance of individuals. This article is part of the theme issue ‘The evolutionary significance of variation in metabolic rates’. 
PY 2024
PD FEB
SO Philosophical Transactions Of The Royal Society B-biological Sciences
SN 0962-8436
PU The Royal Society
VL 379
IS 1896
UT 001137463800004
DI 10.1098/rstb.2022.0483
ID 98205
ER

EF