FN Archimer Export Format
PT J
TI Exploring the Exopolysaccharide Production Potential of Bacterial Strains Isolated from Tunisian Blue Crab Portunus segnis Microbiota
BT 
AF Migaou, Mariem
   Macé, Sabrina
   Maalej, Hana
   Marchand, Laetitia
   Bonnetot, Sandrine
   Noël, Cyril
   Sinquin, Corinne
   Jérôme, Marc
   Zykwinska, Agata
   Colliec-Jouault, Sylvia
   Maaroufi, Raoui Mounir
   Delbarre-Ladrat, Christine
AS 1:1,2;2:2;3:3;4:2;5:2;6:4;7:2;8:2;9:2;10:2;11:1;12:2;
FF 1:;2:PDG-RBE-MASAE-LEMMMB;3:;4:PDG-RBE-MASAE-LEMMMB;5:PDG-RBE-MASAE-LEMMMB;6:PDG-IRSI-SEBIMER;7:PDG-RBE-MASAE-LEMMMB;8:PDG-RBE-MASAE-LEMMMB;9:PDG-RBE-MASAE-LEMMMB;10:PDG-RBE-MASAE-LEMMMB;11:;12:PDG-RBE-MASAE-LEMMMB;
C1 Laboratory of Genetics, Biodiversity & Valorisation of Bioresources, Higher Institute of Biotechnology of Monastir, University of Monastir, Ave Tahar Haddad, BP74, Monastir 5000, Tunisia
   Ifremer, MASAE Microbiologie Aliment Santé Environnement, F-44000 Nantes, France
   Laboratory of Biodiversity and Valorization of Arid Areas Bioresources, Faculty of Sciences, University of Gabès, Erriadh, Zrig, Gabès 6072, Tunisia
   Ifremer, IRSI, SeBiMER Service de Bioinformatique de l’Ifremer, F-29280 Plouzané, France
C2 UNIV MONASTIR, TUNISIA
   IFREMER, FRANCE
   UNIV GABES, TUNISIA
   IFREMER, FRANCE
SI NANTES
   BREST
SE PDG-RBE-MASAE-LEMMMB
   PDG-IRSI-SEBIMER
IN WOS Ifremer UPR
   DOAJ
   copubli-int-hors-europe
   copubli-sud
IF 4.6
TC 0
UR https://archimer.ifremer.fr/doc/00876/98817/108441.pdf
   https://archimer.ifremer.fr/doc/00876/98817/108442.zip
LA English
DT Article
DE ;Portunus segnis;16S rDNA sequencing;microbiota;bacteria;exopolysaccharide
AB The blue crab (BC) Portunus segnis is considered an invasive species colonizing Tunisian coasts since 2014. This work aims to explore its associated bacteria potential to produce anionic exopolysaccharides (EPSs) in order to open up new ways of valorization. In this study, different BC samples were collected from the coastal area of Sfax, Tunisia. First, bacterial DNA was extracted from seven different fractions (flesh, gills, viscera, carapace scraping water, and three wastewaters from the production plant) and then sequenced using the metabarcoding approach targeting the V3-V4 region of the 16S rDNA to describe their microbiota composition. Metabarcoding data showed that the dominant bacterial genera were mainly Psychrobacter, Vagococcus, and Vibrio. In parallel, plate counting assays were performed on different culture media, and about 250 bacterial strains were isolated and identified by sequencing the 16S rDNA. EPS production by this new bacterial diversity was assessed to identify new compounds of biotechnological interest. The identification of the bacterial strains in the collection confirmed the dominance of Psychrobacter spp. strains. Among them, 43 were identified as EPS producers, as revealed by Stains-all dye in agarose gel electrophoresis. A Buttiauxella strain produced an EPS rich in both neutral sugars including rare sugars such as rhamnose and fucose and uronic acids. This original composition allows us to assume its potential for biotechnological applications and, more particularly, for developing innovative therapeutics. This study highlights bacterial strains associated with BC; they are a new untapped source for discovering innovative bioactive compounds for health and cosmetic applications, such as anionic EPS. 
PY 2024
PD FEB
SO Molecules
SN 1420-3049
PU MDPI AG
VL 29
IS 4
UT 001177116500001
DI 10.3390/molecules29040774
ID 98817
ER

EF