FN Archimer Export Format
PT J
TI Substantial gene expression shifts during larval transitions in the pearl oyster Pinctada margaritifera
BT 
AF DESTANQUE, Thibault
   Le Luyer, Jeremy
   Quillien, Virgile
   Sham Koua, Manaarii
   AUFFRET, Pauline
   KY, Chin-Long
AS 1:1;2:1,2;3:1,2;4:1;5:1,3;6:1,4;
FF 1:PDG-RBE-RMPF;2:PDG-RBE-PHYTNESS;3:PDG-RBE-PHYTNESS;4:PDG-RBE-RMPF;5:PDG-IRSI-SEBIMER;6:PDG-RBE-IHPE;
C1 Ifremer, IRD, Institut Louis‐Malardé, Univ Polynésie française EIO Polynésie française ,France
   Ifremer, CNRS, IRD, LEMAR Univ Brest Brest, France
   Ifremer, Sebimer Brest ,France
   Ifremer, UMR 5244 Interactions Hôtes‐Pathogènes‐Environnements Universités de Montpellier Montpellier ,France
C2 IFREMER, FRANCE
   IFREMER, FRANCE
   IFREMER, FRANCE
   IFREMER, FRANCE
SI TAHITI
   BREST
   MONTPELLIER
SE PDG-RBE-RMPF
   PDG-RBE-PHYTNESS
   PDG-IRSI-SEBIMER
   PDG-RBE-IHPE
UM LEMAR
   IHPE
   EIO
IF 2.2
TC 0
UR https://archimer.ifremer.fr/doc/00876/98828/108471.pdf
   https://archimer.ifremer.fr/doc/00876/98828/108472.png
   https://archimer.ifremer.fr/doc/00876/98828/108473.xlsx
   https://archimer.ifremer.fr/doc/00876/98828/108474.csv
LA English
DT Article
DE ;biomineralization;environmental sensing;immune system;larval development;Pinctada margaritifera;RNA-seq
AB Early development stages in marine bivalve are critical periods where larvae transition from pelagic free‐life to sessile mature individuals. The successive metamorphosis requires the expression of key genes, the functions of which might be under high selective pressure, hence understanding larval development represents key knowledge for both fundamental and applied research. Phenotypic larvae development is well known, but the underlying molecular mechanisms such as associated gene expression dynamic and molecular cross‐talks remains poorly described for several nonmodel species, such as P. margaritifera. We designed a whole transcriptome RNA‐sequencing analysis to describe such gene expression dynamics following four larval developmental stages:  d‐shape, Veliger, Umbo and Eye‐spot. Larval gene expression and annotated functions drastically diverge. Metabolic function (gene expression related to lipid, amino acid and carbohydrate use) is highly upregulated in the first development stages, with increasing demand from  d‐shape to umbo. Morphogenesis and larval transition are partly ordered by Thyroid hormones and Wnt signaling. While larvae shells show some similar characteristic to adult shells, the cause of initialization of biomineralization differ from the one found in adults. The present study provides a global overview of Pinctada margaritifera larval stages transitioning through gene expression dynamics, molecular mechanisms and ontogeny of biomineralization, immune system, and sensory perception processes. 
PY 2024
PD MAR
SO Journal of Experimental Zoology Part B: Molecular and Developmental Evolution
SN 1552-5007
PU Wiley
VL 342
IS 2
BP 76
EP 84
DI 10.1002/jez.b.23243
ID 98828
ER

EF