FN Archimer Export Format
PT J
TI Evolutionary and functional insights into the Ski2-like helicase family in Archaea: a comparison of Thermococcales ASH-Ski2 and Hel308 activities
BT 
AF Batista, Manon
   Langendijk-Genevaux, Petra
   Kwapisz, Marta
   Canal, Isabelle
   Phung, Duy Khanh
   Plassart, Laura
   Capeyrou, Régine
   Moalic, Yann
   Jebbar, Mohamed
   Flament, Didier
   Fichant, Gwennaele
   Bouvier, Marie
   Clouet-d’Orval, Béatrice
AS 1:1;2:2;3:1;4:1;5:1;6:1;7:1;8:3;9:3;10:4;11:2;12:1;13:1;
FF 1:;2:;3:;4:;5:;6:;7:;8:;9:;10:PDG-REM-BEEP-LMEE;11:;12:;13:;
C1 MCD, Centre de Biologie Intégrative (CBI), CNRS, Université de Toulouse , UT3, Toulouse, France
   LMGM, Centre de Biologie Intégrative (CBI), CNRS, Université de Toulouse , UT3, Toulouse, France
   Univ Brest, CNRS, Ifremer, UMR6197 Biologie et Ecologie des Ecosystèmes marins Profonds , F-29280 Plouzané, France
   Univ Brest, CNRS, Ifremer, UMR6197 Biologie et Ecologie des Ecosystèmes marins Profonds , F-29280 Plouzané, France
C2 UNIV TOULOUSE, FRANCE
   UNIV TOULOUSE, FRANCE
   UBO, FRANCE
   IFREMER, FRANCE
SI BREST
SE PDG-REM-BEEP-LMEE
UM BEEP-LM2E
IN WOS Ifremer UMR
   WOS Cotutelle UMR
   DOAJ
   copubli-france
   copubli-univ-france
TC 0
UR https://archimer.ifremer.fr/doc/00883/99491/109558.pdf
   https://archimer.ifremer.fr/doc/00883/99491/109559.zip
LA English
DT Article
AB RNA helicases perform essential housekeeping and regulatory functions in all domains of life by binding and unwinding RNA molecules. The Ski2-like proteins are primordial helicases that play an active role in eukaryotic RNA homeostasis pathways, with multiple homologs having specialized functions. The significance of the expansion and diversity of Ski2-like proteins in Archaea, the third domain of life, has not yet been established. Here, by studying the phylogenetic diversity of Ski2-like helicases among archaeal genomes and the enzymatic activities of those in Thermococcales, we provide further evidence of the function of this protein family in archaeal metabolism of nucleic acids. We show that, in the course of evolution, ASH-Ski2 and Hel308-Ski2, the two main groups of Ski2-like proteins, have diverged in their biological functions. Whereas Hel308 has been shown to mainly act on DNA, we show that ASH-Ski2, previously described to be associated with the 5′-3′ aRNase J exonuclease, acts on RNA by supporting an efficient annealing activity, but also an RNA unwinding with a 3′-5′ polarity. To gain insights into the function of Ski2, we also analyse the transcriptome of Thermococcus barophilus ΔASH-Ski2 mutant strain and provide evidence of the importance of ASH-Ski2 in cellular metabolism pathways related to translation. 
PY 2024
PD JAN
SO Nar Genomics And Bioinformatics
SN 2631-9268
PU Oxford University Press (OUP)
VL 6
IS 1
UT 001186356800001
DI 10.1093/nargab/lqae026
ID 99491
ER

EF