Highlighting of quorum sensing lux genes and their expression in the hydrothermal vent shrimp Rimicaris exoculata ectosymbiontic community. Possible use as biogeographic markers

Type Publication
Publication date 2017-03
Language English
Copyright 2017 Le Bloa et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Author(s) Le Bloa Simon1, 2, 3, Durand Lucile1, Cueff-Gauchard Valerie1, 2, 3, Le Bars Josiane1, 2, 3, Taupin Laure4, Marteau Charlotte4, Bazire Alexis4, Cambon-Bonavita Marie-Anne1, 2, 3
Affiliation(s) 1 : IFREMER, Lab Microbiol Environm Extremes, UMR Ifremer CNRS UBO 6197, Ctr Bretagne,REM EEP LM2E, Plouzane, France.
2 : Univ Brest, Lab Microbiol Environm Extremes, UMR Ifremer CNRS UBO 6197, Technopole Iroise,4 Pl Nicolas Copernic, Plouzane, France.
3 : CNRS, Lab Microbiol Environm Extremes, UMR Ifremer CNRS UBO 6197, Technopole Iroise,4 Pl Nicolas Copernic, Plouzane, France.
4 : Univ Bretagne Sud, EA 3884, LBCM, Rue St Maude, Lorient, France.
Source Plos One (1932-6203) (Public Library Science), 2017-03 , Vol. 12 , N. 3 , P. e0174338 (1-19)
DOI 10.1371/journal.pone.0174338
Abstract

Rimicaris exoculata is a caridean shrimp that dominates the fauna at several hydrothermal vent sites of the Mid-Atlantic Ridge. It has two distinct and stable microbial communities. One of these epibiontic bacterial communities is located in the shrimp gut and has a distribution and role that are poorly understood. The second colonizes its enlarged gill chamber and is involved in host nutrition. It is eliminated after each molt, and has colonization processes reminiscent of those of a biofilm. The presence and expression of genes usually involved in quorum sensing (QS) were then studied. At four sites, Rainbow, TAG, Snake Pit and Logatchev, two lux genes were identified in the R. exoculata epibiontic community at different shrimp molt stages and life stages. RT-PCR experiments highlighted lux gene expression activity at TAG, Snake Pit and Rainbow vent sites. Their potential QS activity and their possible roles in epibiont colonization processes are discussed. Moreover, phylogenetic analysis has shown the presence of three clades for luxS (Epsilonproteobacteria) and four clades for luxR (Gammaproteobacteria) genes, each clade being restricted to a single site. These genes are more divergent than the 16S rRNA one. They could therefore be used as biogeographical genetic markers.

Full Text
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Publisher's official version 19 1 MB Open access
S1 File. The SI File is a more detailed description of bioinformatic studies followed by extraction and quantification of N-acylhomoserine lactones (AHLs) description. 11 219 KB Open access
S1 Fig. LuxS protein sequences alignment. 5 MB Open access
S2 Fig. LuxR protein analysis 8 MB Open access
S3 Fig. Chromatograph of N-acylhomoserine lactone extraction. 1 MB Open access
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How to cite 

Le Bloa Simon, Durand Lucile, Cueff-Gauchard Valerie, Le Bars Josiane, Taupin Laure, Marteau Charlotte, Bazire Alexis, Cambon-Bonavita Marie-Anne (2017). Highlighting of quorum sensing lux genes and their expression in the hydrothermal vent shrimp Rimicaris exoculata ectosymbiontic community. Possible use as biogeographic markers. Plos One, 12(3), e0174338 (1-19). Publisher's official version : http://doi.org/10.1371/journal.pone.0174338 , Open Access version : http://archimer.ifremer.fr/doc/00377/48841/