Determination of peptaibol trace amounts in marine sediments by liquid chromatography/electrospray ionization-ion trap-mass spectrometry

Extraction followed by reverse phase liquid chromatography (LC)/electrospray ionization-ion trap-mass spectrometry (ESI-IT-MS) analysis has been successfully developed for the determination of peptaibols, fungal toxic metabolites, in marine sediments. Spiking experiments showed that the mean recovery of target compounds exceeded 85% at a spiking level of 10 ng/g of sediment (wet weight). Detection and quantification limits were 250 and 830 pg/g of sediment, respectively. The method developed constituted the first sensitive assay for quantification of peptaibol trace amounts in a natural environment. A concentration of 5 ng/g in sediment samples collected from Fier d'Ars was found.

Keyword(s)

Matched calibration, Matrix, Electrospray ionization ion trap mass spectrometry ESI IT MS, Marine fungal contamination, Trichoderma sp., Fungal peptide metabolites

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Poirier Laurence, Amiard Jean-Claude, Mondeguer Florence, Quiniou Francoise, Ruiz Nicolas, Pouchus Yves Francois, Montagu Monique (2007). Determination of peptaibol trace amounts in marine sediments by liquid chromatography/electrospray ionization-ion trap-mass spectrometry. Journal of Chromatography A. 1160 (1-2). 106-113. https://doi.org/10.1016/j.chroma.2007.04.006, https://archimer.ifremer.fr/doc/00000/2990/

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