The spatial structure of demersal fish assemblages on the continental shelf of the NW Mediterranean Sea was examined on a large scale between the Straits of Gibraltar and the Gulf of Lions. In contrast to its predominant role in the spatial distribution of species composition, described worldwide, it seems depth has only a limited effect on the distribution patterns of the total abundance and diversity of demersal fishes. In addition, evidence is provided for the division of the whole study area into 3 geographical fish assemblages. The influence of Atlantic inputs and the semi-permanent Almena-Oran hydrographic front play a key role in defining the Alboran Sea as a specific fish fauna area. Moreover, the clear differentiation between 2 distinct assemblages in the Gulf of Lions and the Catalan Sea could be partly linked to the North Balearic front. These results contribute to the current debate on the definition of spatial units for the purpose of demersal fisheries management. They also stress the complexity of developing an ecosystem approach which deals simultaneously with species composition and biodiversity aspects of ecosystems management.