Quantitative analyses on calcareous nannofossil assemblages on high temporal resolution (600-800 years) have been carried out from Core KC01B in the Ionian Sea (Eastern Mediterranean) throughout Marine Isotope Stage (MIS) 13-9, between 500 and 300 ka. This is an interval of considerable climate changes, known as the Mid-Brunhes event, which includes MIS 11, considered as a possible analogue for future interglacial conditions. In the Mediterranean core, the interval is characterized by the dominance of Gephyrocapsa spp., as also known from ocean records. Calcareous nannofossil abundance fluctuations have been interpreted in terms of modification of sea surface primary productivity/water stratification and temperature. Specifically, the increase in abundance of Gephyrocapsa caribbeanica and small Gephyrocapsa coupled with decreases of Florisphaera profunda, Syracosphaera spp. and Rhabdosphaera spp. suggests enhanced primary productivity/mixed surface waters during interglacial stages and at the Terminations, which can be interpreted in terms of interaction between climate changes and mesoscale oceanographic circulation. On the other hand, distributions of Calciosolenia spp., Oolithotus spp. and Umbilicosphaera sibogae, which are in phase with delta O-18 curve showing higher abundances at the lighter values of the marine isotope record, are considered as warm-water indicators. Biotic proxies point to peculiar climate dynamics through the MIS 12-11 transition (i.e. Termination V). High productive, cool, low salinity and turbid surface waters characterize the early MIS 11(421-408 ka) and likely reflect enhanced continental humidity/monsoon activity over North Africa and increased runoff into the basin. A late surface water warming with respect to Termination V is established at about 403 ka, during a period of low insolation forcing, almost coeval with the deposition of sapropel S11. A climate optimum, lasting about 15 ky (403-389 ka), is then recorded during a period of low insolation forcing centred at 398 ka. The later part of MIS 11 is characterized by climate deterioration at about 389 ka. Comparison with results from subtropical to polar Atlantic records suggests a remarkable relation between Mediterranean and Atlantic climate regimes.