Reduction of adhesion properties of Ruditapes philippinarum hemocytes exposed to Vibrio tapetis

Vibrio tapetis is the causative agent of brown ring disease (BRD), which affects a species of clam, Ruditapes philippinarum. After incubation with V. tapetis, hemocytes lose filopods and become rounded, indicating cytotoxic activity of the bacterium. To rapidly quantify this cytotoxicity, a flow-cytometry test was developed based on the capacity of V tapetis to inhibit adhesion of clam hemocytes to plastic. Several bacteria:hemocyte ratios, the cytotoxicity of other Vibrio spp. pathogenic to bivalves, and that of various V. tapetis isolates were tested. Inhibition of adherence is detectable with as few as 5 bacteria per hemocyte. The greater cytotoxic activity of V tapetis compared to that of V splendidus and V pectenicida suggests a specific pathogenicity of V. tapetis to R. philippinarum hemocytes. Although all V. tapetis isolates inhibited adhesion, significant variations in cytotoxicity among isolates was demonstrated.

Keyword(s)

cytotoxicity, flow cytometry, Vibrio tapetis, Ruditapes philippinarum, bivalve hemocytes, pathogenicity

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Choquet Gwenaelle, Soudant P, Lambert C, Nicolas Jean-Louis, Paillard C (2003). Reduction of adhesion properties of Ruditapes philippinarum hemocytes exposed to Vibrio tapetis. Diseases Of Aquatic Organisms. 57 (1-2). 109-116. https://doi.org/10.3354/dao057109, https://archimer.ifremer.fr/doc/00000/10761/

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