Oligonucleotide quartz crystal microbalance sensor for the microalgae Alexandrium minutum (Dinophyceae)

We report the immobilization on a gold surface of a 20-base DNA probe labeled with disulfide group and on the selective hybridization with the complementary 20-base DNA strand. The oligonucleotide probe is the complementary strand of a partial sequence of the gene encoding for a large ribosomal RNA sub-unit which is a coding sequence of Alexandrium minutum DNA, a microalgae that produces neurotoxins responsible for paralytic shellfish poisoning on European and Asian coasts. The kinetics of DNA probe immobilization and hybridization were monitored in situ by using a 27 MHz quartz crystal microbalance under controlled hydrodynamic conditions. The frequency of the setup is stable to within a few hertz, corresponding to the nanogram, scale, for 3 h and makes it possible to follow frequency change from immobilization of the probe to hybridization of the complementary DNA target. This setup constitutes a biosensor, which is sensitive and selective, and the hybridization ratio between hybridized complementary DNA and immobilized DNA probes is 47%.

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DNA, Alexandrium minutum, Microbalance

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Lazerges M, Perrot H, Antoine Elisabeth, Defontaine Audrey, Compere Chantal (2006). Oligonucleotide quartz crystal microbalance sensor for the microalgae Alexandrium minutum (Dinophyceae). Biosensors and Bioelectronics. 21 (7). 1355-1358. https://doi.org/10.1016/j.bios.2005.05.013, https://archimer.ifremer.fr/doc/00000/1354/

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