Building of an immunosensor: How can the composition and structure of the thiol attachment layer affect the immunosensor efficiency?
Immunosensors, based on the immobilization of a model rabbit antibody on mixed self-assembled monolayers and Protein A as a linking agent on gold transducers, were elaborated and characterized at each step by modulated polarization-infrared spectroscopy (PM-IRRAS) and occasionally by atomic force microscopy (AFM) and quartz crystal microbalance (QCM). By testing two different mixed SAMs comprising 11-mercaptoundecanoic acid (MUA), together with either decanethiol (C9CH3) or mercaptohexanol (C6OH), the role of the chemical composition and structure of the antibody attachment layer upon the sensor performance was demonstrated. (c) 2006 Elsevier B.V. All rights reserved.
Keyword(s)
QCM, IRRAS, Thiol attachment, Immunosensor, Mixed SAM' s
Briand Elisabeth, Salmain Michèle, Henry Jean-Marie, Perrot Hubert, Compere Chantal, Pradier Claire-Marie (2006). Building of an immunosensor: How can the composition and structure of the thiol attachment layer affect the immunosensor efficiency?. Biosensors and Bioelectronics. 22 (3). 440-448. https://doi.org/10.1016/j.bios.2006.05.018, https://archimer.ifremer.fr/doc/00000/1988/
