Evidence for chemoautotrophic symbiosis in a Mediterranean cold seep clam (Bivalvia : Lucinidae): comparative sequence analysis of bacterial 16S rRNA, APS reductase and RubisCO genes
| Type | Article | ||||||||
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| Date | 2007 | ||||||||
| Language | English | ||||||||
| Author(s) | Duperron Sebastien1, 2, 3, Fiala Medioni Aline4, Caprais Jean-Claude2, Olu Karine 2, Sibuet Myriam2 |
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| Affiliation(s) | 1 : Univ Paris 06, UMR 7138, F-75005 Paris, France. 2 : IFREMER, Ctr Brest, Lab Environm Profond, F-29280 Plouzane, France. 3 : Max Planck Inst Marine Microbiol, Bremen, Germany. 4 : Univ Paris 06, Observ Oceanol, Banyuls sur Mer, France. |
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| Source | FEMS Microbiology Ecology (0168-6496) (Blackwell science), 2007 , Vol. 59 , N. 1 , P. 64-70 | ||||||||
| DOI | 10.1111/j.1574-6941.2006.00194.x | ||||||||
| WOS© Times Cited | 57 | ||||||||
| Keyword(s) | Eastern Mediterranean, Cold seeps, Lucinoma, Lucinidae, Sulphide oxidizing bacteria, Symbiosis | ||||||||
| Abstract | Symbioses between lucinid clams (Bivalvia: Lucinidae) and autotrophic sulphide-oxidizing bacteria have mainly been studied in shallow coastal species, and information regarding deep-sea species is scarce. Here we study the symbiosis of a clam, resembling Lucinoma kazani, which was recently collected in sediment cores from new cold-seep sites in the vicinity of the Nile deep-sea fan, eastern Mediterranean, at depths ranging from 507 to 1691 m. A dominant bacterial phylotype, related to the sulphide-oxidizing symbiont of Lucinoma aequizonata, was identified in gill tissue by comparative 16S rRNA gene sequence analysis. A second phylotype, related to spirochete sequences, was identified twice in a library of 94 clones. Comparative analyses of gene sequences encoding the APS reductase alpha subunit and ribulose-1,5-bisphosphate carboxylase oxygenase support the hypothesis that the dominant symbiont can perform sulphide oxidation and autotrophy. Transmission electron micrographs of gills confirmed the dominance of sulphide-oxidizing bacteria, which display typical vacuoles, and delta C-13 values measured in gill and foot tissue further support the hypothesis for a chemoautotrophic-sourced host carbon nutrition. | ||||||||
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