Marine water quality assessment using transplanted oyster larvae

Type Article
Date 2007
Language English
Author(s) Quiniou Francoise1, Damiens G2, Gnassia Barelli M2, Geffard A3, Mouneyrac C4, 5, Budzinski H6, Romeo Michèle2
Affiliation(s) 1 : INRA, UNSA, ROSE, Fac Sci,UMR 1112, F-06108 Nice 2, France.
2 : IFREMER, Ctr Brest, Dept Biogeochim & Ecotoxicol, F-29280 Plouzane, France.
3 : EA 2069 URVVC, Laboratoire d'Eco-Toxicologie, Faculté des Sciences Exactes et Naturelles, Moulin de la Housse BP 1039, 51687 REIMS Cedex, France
4 : CEREA, IBEA UCO, F-49008 Angers 01, France.
5 : SMAB ISOMER, Fac Sci, Serv Ecotoxicol, F-44322 Nantes 3, France.
6 : Univ Bordeaux 1, LPTC, F-3405 Talence, France.
Source Environment International (0160-4120) (Elsevier), 2007 , Vol. 33 , N. 1 , P. 27-33
DOI 10.1016/j.envint.2006.06.020
WOS© Times Cited 31
Keyword(s) PAHs, Trace metals, Biomarkers, Active bio monitoring, Crassostrea gigas larvae
Abstract Active bio-monitoring in terms of biornarkers was attempted using Crassostrea gigas larvae produced in the laboratory and transplanted using special containers to two sites at the entrance (A) and inner part (P) of the harbour of Arcachon (French Atlantic Coast). The larvae were kept in the medium for 48 h. Their physiological status and their biomarker levels : acetylcholinesterase AChE, catalase CAT and glutathione S-transferase GST activities were determined together with metallothionein Wand Thiobarbituric Acid Reactive Substances TBARS concentrations. Copper and PAH (polycyclic aromatic hydrocarbon) concentrations were determined in the exposed larvae and in the sediments collected under the containers. Cadmium, lead and zinc could be also analyzed in the sediments. Toxicity tests demonstrate that the larvae are in better physiological conditions in A compared to P. Larvae transplanted in the inner harbour (P) present relatively high GST activity (869.1 +/- 39.3 nmol min(-1) mg protein(-1)), TBARS (2.74 +/- 0.19 nmol mg protein(-1)), compared to those exposed at the harbour entrance (A). Copper measured in the sediments (65 +/- 1 mg kg(-1) d.w.) collected under the cages at P is higher than at A. Larvae placed in A present higher total PAH concentrations compared to the inner part. The data tend to reveal a lower copper and higher PAH contamination in A than in P. Therefore larvae, developing in the natural medium, show different responses according to their immersion sites. These responses, obtained within 48 h, may be related to the chemical contamination of the environment and may be used for seawater quality assessment in future studies. (c) 2006 Elsevier Ltd. All rights reserved.
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