Characterization of extracellular polymers synthesized by tropical intertidal biofilm bacteria
| Type | Article | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Date | 2007 | ||||||||
| Language | English | ||||||||
| Author(s) | Ortega Morales Benjamín Otto1, Santiago Garcia J.L1, Chan Bacab M.J.1, Moppert Xavier2, Miranda Tello E3, Fardeau M.L.3, Carrero J.C.4, Bartolo Perez P.1, 5, Valadez Gonzalez A6, Guezennec Jean2 | ||||||||
| Affiliation(s) | 1 : Univ Autonoma Campeche, Dept Microbiol Ambiental & Biotecnol, Programa Corros Golfo Mexico, Campeche 24030, Mexico. 2 : IFREMER, Plouzane, France. 3 : Univ Mediterranee, Inst Rech Dev UR101, IFR BAIM, Marseille 09, France. 4 : Univ Nacl Autonoma Mexico, Dept Immunol, Inst Invest Biomed, Mexico City 04510, DF, Mexico. 5 : CINVESTAV, IPN Merida, Dept Fis Aplicada, Yucatan, Mexico. 6 : Ctr Invest Cient Yucatan, Dept Mat, Yucatan, Mexico. |
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| Source | Journal of Applied Microbiology (1364-5072) (Blackwell science), 2007 , Vol. 102 , N. 1 , P. 254-264 | ||||||||
| DOI | 10.1111/j.1365-2672.2006.03085.x | ||||||||
| WOS© Times Cited | 65 | ||||||||
| Keyword(s) | Tropical intertidal biofilm bacteria, Surfactants, Microbacterium sp, Exopolymers, Bacillus sp, Anionic | ||||||||
| Abstract | Aims: This study was performed to determine the potential of tropical intertidal biofilm bacteria as a source of novel exopolymers (EPS). Methods and Results: A screening procedure was implemented to detect EPS-producing biofilm bacteria. Isolates MC3B-10 and MC6B-22, identified respectively as a Microbacterium species and Bacillus species by 16S rDNA and cellular fatty acids analyses, produced different EPS, as evidenced by colorimetric and gas chromatographic analyses. The polymer produced by isolate MC3B-10 displays significant surfactant activity, and may chelate calcium as evidenced by spectroscopic analysis. Conclusions: Polymer MC3B-10 appears to be a glycoprotein, while EPS MC6B-22 seems to be a true polysaccharide dominated by neutral sugars but with significant concentrations of uronic acids and hexosamines. EPS MC3B-10 possesses a higher surfactant activity than that of commercial surfactants, and given its anionic nature, may chelate cations thus proving useful in bioremediation. The chemical composition of polymer MC6B-22 suggests its potential biomedical application in tissue regeneration. Significance and Impact of the Study: This is the first report of a Microbacterium species producing EPS with surfactant properties, which expands our knowledge of the micro-organisms capable of producing these biomolecules. Furthermore, this work shows that tropical intertidal environments are a nonpreviously recognized habitat for bioprospecting EPS-producing bacteria, and that these molecules might be involved in ecological roles protecting the cells against dessication. | ||||||||
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