Molecular characterisation of prolactin and analysis of extrapituitary expression in the European sea bass Dicentrarchus labrax under various salinity conditions

Type Article
Date 2007-03
Language English
Author(s) Boutet Isabelle1, 2, Lorin Nebel C1, De Lorgeril JulienORCID1, 2, Guinand B1
Affiliation(s) 1 : Univ Montpellier 2, IFREMER, CNRS, UMR 5171,Lab Genome Populat Interact Adaptat, F-34095 Montpellier 5, France.
Source Comparative Biochemistry and Physiology Part D: Genomics and Proteomics (1744-117X) (Elsevier), 2007-03 , Vol. 2 , N. 1 , P. 74-83
DOI 10.1016/j.cbd.2006.12.002
WOS© Times Cited 18
Keyword(s) Prolactin, Phylogenctic analysis, MRNA expression, Osmoregulation, Intestine, Gill, Dicentrarchuslabrax
Abstract dAlthough prolactin has been demonstrated to be the main hormone involved in adaptation to dilute media in several freshwater teleosts, few studies have been conducted in marine teleosts. In the Mediterranean, the sea bass Dicentrarchus labrax inhabits environments ranging from the open sea to coastal lagoons, where salinity varies greatly. We characterised the prolactin (prl) gene and analysed its expression in two organs (gill and intestine) in D. labrax acclimated to either freshwater or seawater. A 2819 bp long sequence encompassing the prl gene and a part (282 bp) of the promoter were identified, and these comprised 5 coding exons separated by 4 introns. Prolactin was similarly expressed in fresh- and seawater adapted fish, although expression in gills was significantly greater than in the intestine. Nonetheless, individuals unable to successfully regulate osmotic balance in freshwater presented overall low expression rates. Results are discussed according to the mechanism of sea bass adaptation in the wild and to their life cycle between open sea and lagoons. Finally, a phylogenetic analysis indicated that teleosts are not branched according to their life-history features (e.g. seawater vs. freshwater habitats), and no signature of positive selection was detected across the phylogeny of the prl gene in teleosts. (c) 2006 Elsevier Inc. All rights reserved.
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Boutet Isabelle, Lorin Nebel C, De Lorgeril Julien, Guinand B (2007). Molecular characterisation of prolactin and analysis of extrapituitary expression in the European sea bass Dicentrarchus labrax under various salinity conditions. Comparative Biochemistry and Physiology Part D: Genomics and Proteomics, 2(1), 74-83. Publisher's official version : https://doi.org/10.1016/j.cbd.2006.12.002 , Open Access version : https://archimer.ifremer.fr/doc/00000/2537/