Changes in biochemical and hemocyte parameters of the pacific oysters Crassostrea gigas fed T-Iso supplemented with lipid emulsions rich in eicosapentaenoic acid

Type Article
Date 2007-05
Language English
Author(s) Delaporte Maryse2, Chu Fu-Lin3, Langdon Chris4, Moal Jeanne2, Lambert Christophe1, Samain Jean-Francois2, Soudant Philippe1
Affiliation(s) 1 : Univ Bretagne Occidentale, LEMAR, UMR 6539, Inst Univ Europeen Mer,Lab Sci Environm Marin, F-29280 Plouzane, France.
2 : IFREMER, Ctr Brest, UMR Ecophysiol & Physiol Mollusques Marins, F-29280 Plouzane, France.
3 : Virginia Inst Marine Sci, Sch Marine Sci, Gloucester Point, VA 23062 USA.
4 : Oregon State Univ, Coastatl Oregon Marine Expt Stn, Newport, OR 97365 USA.
Source Journal of Experimental Marine Biology and Ecology (0022-0981) (Elsevier), 2007-05 , Vol. 343 , N. 2 , P. 261-275
DOI 10.1016/j.jembe.2006.12.021
WOS© Times Cited 20
Keyword(s) Polyunsaturated fatty acids, Immunology, Hemocytes, Eicosapentaenoic acid 20 : 5n 3, Crassostrea gigas, Bivalves
Abstract The aim of this study was to assess the effect of dietary eicosapentaenoic acid (20:5n-3) on hemocyte parameters such as hemocyte concentration, phagocytosis, and non-stimulated reactive oxygen species (ROS) production in Pacific oysters Crassostrea gigas, as well on proximate biochemical and fatty acid compositions. One-year-old oysters (C. gigas) were fed T-Isochrysis aff. galbana(T-Iso), which is low in 20:5n-3, either alone or with supplements of a lipid emulsion rich in 20:5n-3 at 1%, 10% or 50% (dry weight of the algal ration) for up to 7 weeks. Changes in gill fatty acid composition demonstrated that the lipid emulsion was well ingested by oysters during the dietary conditioning. Biochemical analysis indicated that oysters fed supplements of 50% and, to a lesser extent, 10% lipid emulsions had a higher total lipid content compared with oysters fed other diets, suggesting a more advanced reproductive status for the oysters fed high doses of lipid emulsion. Moreover, some oysters in these two treatment groups spawned during the last three weeks of the seven-week feeding experiment. Lipid supplements had a significant influence on hemocyte concentration, phagocytic index and non-stimulated hemocyte ROS production. After 4 weeks, highest hemocyte concentrations were found in oysters fed on a supplement of 50% lipid emulsion compared with those fed on other diets but the hemocytes derived from these oysters had the lowest short-term phagocytic index. After 7 weeks of dietary conditioning, the ROS production in non-stimulated hemocytes of oysters fed 10% and 50% lipid emulsion declined. These results suggested that 20:5n-3, and perhaps its eicosanoid metabolites, affected oyster hemocyte functions; however, the reproductive status of oysters may also have interfered with the 20:5n-3 dietary effect. (c) 2007 Elsevier B.V. All rights reserved.
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Delaporte Maryse, Chu Fu-Lin, Langdon Chris, Moal Jeanne, Lambert Christophe, Samain Jean-Francois, Soudant Philippe (2007). Changes in biochemical and hemocyte parameters of the pacific oysters Crassostrea gigas fed T-Iso supplemented with lipid emulsions rich in eicosapentaenoic acid. Journal of Experimental Marine Biology and Ecology, 343(2), 261-275. Publisher's official version : https://doi.org/10.1016/j.jembe.2006.12.021 , Open Access version : https://archimer.ifremer.fr/doc/00000/2604/