Monoclonal antibody against a gill Rickettsiales-like organism of Pecten maxiumus (Bivalvia): application to indirect immunofluorescence diagnosis

Using hybridoma technology, monoclonal antibodies (MABs) were prepared against a Rickettsiales-like organism (RLO) from the gills of the Saint-Jacques scallop Pecten maximus. The 5D7 MAB was then selected as a specific reagent suitable for an indirect immunofluorescence assay (IIFA) and evaluated for RLO diagnosis on P. maxiumus gill smears. Monoclonal antibody 5D7 showed no cross-reactivity with an RLO associated with a related pectinid species Chlamys opercularis

Keyword(s)

Diagnosis, Rickettsiales like organism, Monoclona antibody, Pecten maximus

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Le Gall Gyslaine, Mourton Chantal, Boulo Viviane, Paolucci Francis, Pau Bernard, Mialhe Eric (1992). Monoclonal antibody against a gill Rickettsiales-like organism of Pecten maxiumus (Bivalvia): application to indirect immunofluorescence diagnosis. Diseases of aquatic organisms. 14. 213-217. https://archimer.ifremer.fr/doc/00000/2762/

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