Primers and polymerase chain reaction conditions for DNA barcoding teleost fish based on the mitochondrial cytochrome b and nuclear rhodopsin genes
|Author(s)||Sevilla Rafael G.1, Diez Amalia1, Noren Michael2, Mouchel Olivier3, Jerome Marc3, Verrez-Bagnis Veronique3, Van Pelt Hilde4, Favre Krey Laurence5, Krey Grigorios5, Bautista José M.1|
|Affiliation(s)||1 : Univ Complutense Madrid, Dept Biochem & Mol Biol 4, Fac Vet, E-28040 Madrid, Spain.
2 : Swedish Museum Nat Hist, SE-10405 Stockholm, Sweden.
3 : French Res Inst Exploitat Sea IFREMER, F-44311 Nantes, France.
4 : Netherlands Inst Fisheries Res, NL-1970 AB Ijmuiden, Netherlands.
5 : Natl Agr Res Fdn Fisheries Res Inst, GR-64007 Nea Peramos, Kavala, Greece.
|Source||Molecular Ecology Notes (1471-8278) (Blackwell science), 2007-09 , Vol. 7 , N. 5 , P. 730-734|
|WOS© Times Cited||144|
|Keyword(s)||Teleost, Rhodopsin, PCR primers, Mitochondrial cytochrome b, Fish, DNA barcoding|
|Abstract||This report describes a set of 21 polymerase chain reaction primers and amplification conditions developed to barcode practically any teleost fish species according to their mitochondrial cytochrome b and nuclear rhodopsin gene sequences. The method was successfully tested in more than 200 marine fish species comprising the main Actinopterygii family groups. When used in phylogenetic analyses, its combination of two genes with different evolutionary rates serves to identify fish at the species level. We provide a flow diagram indicating our validated polymerase chain reaction amplification conditions for barcoding and species identification applications as well as population structure or haplotyping analyses, adaptable to high-throughput analyses.|