Primers and polymerase chain reaction conditions for DNA barcoding teleost fish based on the mitochondrial cytochrome b and nuclear rhodopsin genes

Type Article
Date 2007-09
Language English
Author(s) Sevilla Rafael G.1, Diez Amalia1, Noren Michael2, Mouchel Olivier3, Jerome Marc3, Verrez-Bagnis VeroniqueORCID3, Van Pelt Hilde4, Favre Krey Laurence5, Krey Grigorios5, Bautista José M.1
Affiliation(s) 1 : Univ Complutense Madrid, Dept Biochem & Mol Biol 4, Fac Vet, E-28040 Madrid, Spain.
2 : Swedish Museum Nat Hist, SE-10405 Stockholm, Sweden.
3 : French Res Inst Exploitat Sea IFREMER, F-44311 Nantes, France.
4 : Netherlands Inst Fisheries Res, NL-1970 AB Ijmuiden, Netherlands.
5 : Natl Agr Res Fdn Fisheries Res Inst, GR-64007 Nea Peramos, Kavala, Greece.
Source Molecular Ecology Notes (1471-8278) (Blackwell science), 2007-09 , Vol. 7 , N. 5 , P. 730-734
DOI 10.1111/j.1471-8286.2007.01863.x
WOS© Times Cited 134
Keyword(s) Teleost, Rhodopsin, PCR primers, Mitochondrial cytochrome b, Fish, DNA barcoding
Abstract This report describes a set of 21 polymerase chain reaction primers and amplification conditions developed to barcode practically any teleost fish species according to their mitochondrial cytochrome b and nuclear rhodopsin gene sequences. The method was successfully tested in more than 200 marine fish species comprising the main Actinopterygii family groups. When used in phylogenetic analyses, its combination of two genes with different evolutionary rates serves to identify fish at the species level. We provide a flow diagram indicating our validated polymerase chain reaction amplification conditions for barcoding and species identification applications as well as population structure or haplotyping analyses, adaptable to high-throughput analyses.
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Sevilla Rafael G., Diez Amalia, Noren Michael, Mouchel Olivier, Jerome Marc, Verrez-Bagnis Veronique, Van Pelt Hilde, Favre Krey Laurence, Krey Grigorios, Bautista José M. (2007). Primers and polymerase chain reaction conditions for DNA barcoding teleost fish based on the mitochondrial cytochrome b and nuclear rhodopsin genes. Molecular Ecology Notes, 7(5), 730-734. Publisher's official version : https://doi.org/10.1111/j.1471-8286.2007.01863.x , Open Access version : https://archimer.ifremer.fr/doc/00000/3034/