||Delaporte Maryse, Moal Jeanne, Soudant Philippe, Lambert Christophe, Pouvreau Stephane, Enriquez Martha, Degremont Lionel, Soletchnik Patrick, Gagnaire Beatrice, Ropert Michel
||National Shellfishery Association (NSA) 2003
||Defense mechanisms, Nutrition, Seasonal variations, Physiology, Crassostrea gigas, Pacific oysters
||In the frame of MOREST project, a common biological material, resulting of a mixture of different families produced in experimental hatchery, was reared in two different environmental fields : Normandy and Charente. Concomitantly, a pool was conditioned at the Ifremer Argenton hatchery with three different algae levels : 4%, 8% and 12% of algal dry weight per oyster dry weight. During the experiments, five immune parameters were studied in parallel with survival rate and reproductive status (stages and intensity). Site location, seasonal variations and experimental diet level clearly influenced oyster immune parameters. Haemocyte counts were higher for oysters reared in Normandy than those reared in Charente and in hatchery. Granulocyte percentage was drastically reduced in hatchery conditions compared to in situ conditions. Moreover, hemocyte activities were also affected by the in situ conditions and dietary treatments in relation to reproductive cycle and mortality events. In example, haemocyte adhesion capacities were more affected by the presence of bacteria during high mortality period.