Evidence of a bactericidal permeability increasing protein in an invertebrate, the Crassostrea gigas Cg-BPI

Type Article
Date 2007-11
Language English
Author(s) Gonzalez Marcelo6, Gueguen YannickORCID1, Destoumieux Garzon Delphine2, Romestand Bernard6, Fievet Julie1, Pugniere M2, Roquet F2, Escoubas Jean-Michel2, Vandenbulcke F3, Levy O4, Saune Laure1, Bulet P5, Bachere Evelyne1
Affiliation(s) 1 : Ifremer, Centre National de la Recherche Scientifique, Université Montpellier 2, Unité Mixte de Recherche 5119, Ecosystèmes Lagunaires, Place E. Bataillon, CC80, 34095 Montpellier Cedex 5, France
2 : Centre National de la Recherche Scientifique, Unité Mixte de Recherche 5236, Centre d'Études d'Agents Pathogènes et Biotechnologie pour la Santé, Institut de Biologie, 34965 Montpellier, France
3 : Université des Sciences et Technologies de Lille, Ecologie Numérique et Ecotoxicologie,59655 Villeneuve d'Ascq, France;
4 : Children's Hospital Boston and Harvard Medical School, Boston, MA 02115
5 : Atheris Laboratories, CP314, 1233 Bernex-Geneva, Switzerland
Source Proceedings of the National Academy of Sciences of the United States of America (0027-8424) (National Academy of Sciences), 2007-11 , Vol. 104 , N. 45 , P. 17759-17764
DOI 10.1073/pnas.0702281104
WOS© Times Cited 95
Keyword(s) Oyster innate immunity, Mollusk, Hemocyte, Epithelia, Antimicrobial
Abstract A cDNA sequence with homologies to members of the LPS-binding protein and bactericidal/permeability-increasing protein (BPI) family was identified in the oyster Crassostrea gigas. The recombinant protein was found to bind LIPS, to display bactericidal activity against Escherichia coli, and to increase the permeability of the bacterial cytoplasmic membrane. This indicated that it is a BPI rather than an LPS-binding protein. By in situ hybridization, the expression of the C gigas BPI (Cg-bpi) was found to be induced in hemocytes after oyster bacterial challenge and to be constitutive in various epithelia of unchallenged oysters. Thus, Cg-bpi transcripts were detected in the epithelial cells of tissues/organs in contact with the external environment (mantle, gills, digestive tract, digestive gland diverticula, and gonad follicles). Therefore, Cg-BPI, whose expression profile and biological properties are reminiscent of mammalian BPIs, may provide a first line of defense against potential bacterial invasion. To our knowledge, this is the first characterization of a BPI in an invertebrate.
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Gonzalez Marcelo, Gueguen Yannick, Destoumieux Garzon Delphine, Romestand Bernard, Fievet Julie, Pugniere M, Roquet F, Escoubas Jean-Michel, Vandenbulcke F, Levy O, Saune Laure, Bulet P, Bachere Evelyne (2007). Evidence of a bactericidal permeability increasing protein in an invertebrate, the Crassostrea gigas Cg-BPI. Proceedings of the National Academy of Sciences of the United States of America, 104(45), 17759-17764. Publisher's official version : https://doi.org/10.1073/pnas.0702281104 , Open Access version : https://archimer.ifremer.fr/doc/00000/3564/