Correlation between detection of a plasmid and high-level virulence of Vibrio nigripulchritudo, a pathogen of the shrimp Litopenaeus stylirostris

Type Article
Date 2008-05
Language English
Author(s) Reynaud Yann2, 3, Saulnier Denis2, Mazel D1, Goarant Cyrille3, Le Roux F1, 2
Affiliation(s) 1 : Inst Pasteur, Unit Plast Genome Bacterien, CNRS, URA 2171, F-75724 Paris, France.
2 : Lab Genet & Pathol, F-17390 Ifremer, La Tremblade, France.
3 : Dept Aquaculture, Noumea 98846, New Caledonia.
Source Applied and environmental microbiology (depuis 1953) (0099-2240) (American society for microbiology), 2008-05 , Vol. 74 , N. 10 , P. 3038-3047
DOI 10.1128/AEM.02680-07
WOS© Times Cited 16
Abstract Vibrio nigripulchritudo, the etiological agent of Litopenaeus stylirostris summer syndrome, is responsible for mass mortalities of shrimp in New Caledonia. Epidemiological studies led to the suggestion that this disease is caused by an emergent group of pathogenic strains. Genomic subtractive hybridization was carried out between two isolates exhibiting low and high virulence. Our subtraction library was constituted of 521 specific fragments; 55 of these were detected in all virulent isolates from our collection (n = 32), and 13 were detected only in the isolates demonstrating the highest pathogenicity (n = 19), suggesting that they could be used as genetic markers for high virulence capacity. Interestingly, 10 of these markers are carried by a replicon of 11.2 kbp that contains sequences highly similar to those of a plasmid detected in Vibrio shilonii, a coral pathogen. The detection of this plasmid was correlated with the highest pathogenicity status of the isolates from our collection. The origin and consequence of this plasmid acquisition are discussed.
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Reynaud Yann, Saulnier Denis, Mazel D, Goarant Cyrille, Le Roux F (2008). Correlation between detection of a plasmid and high-level virulence of Vibrio nigripulchritudo, a pathogen of the shrimp Litopenaeus stylirostris. Applied and environmental microbiology (depuis 1953), 74(10), 3038-3047. Publisher's official version : https://doi.org/10.1128/AEM.02680-07 , Open Access version : https://archimer.ifremer.fr/doc/00000/4523/