Interspecific hybridization in oysters: Restriction enzyme digestion chromosome banding confirms Crassostrea angulata x Crassostrea gigas F1 hybrids

Type Article
Date 2007-05
Language English
Author(s) Leitao A1, Chaves R1, Santos S1, Guedes Pinto H1, Boudry PierreORCID2
Affiliation(s) 1 : UTAD, CGB, Dept Genet & Biotechnol, P-5000911 Vila Real, Portugal.
2 : Stn Inst Rech & Exploitat Mer, Lab Genet & Pathol, F-17390 La Tremblade, France.
Source Journal of Experimental Marine Biology and Ecology (0022-0981) (Elsevier), 2007-05 , Vol. 343 , N. 2 , P. 253-260
DOI 10.1016/j.jembe.2006.12.007
WOS© Times Cited 20
Keyword(s) Interspecific hybrids, In situ restriction enzyme banding, Crassostrea gigas, C. angulata, Chromosome banding
Abstract The taxonomic status of the two commercially important cupped oysters, Crassostrea angulata, the Portuguese oyster (Lamarck, 1819) and Crassostrea gigas, the Japanese oyster (Thunberg, 1793) has long been in question. The recent observation of the hybridization between C. gigas and C. angulata and the production of fertile F1s led us to search for cytogenetic evidence of both parental genomes in the interspecific hybrids. The cytogenetic characterization of the hybrids was performed by the use of restriction endonuclease treatments. This technique has recently shown the potential for individual chromosome identification by banding in oysters. Chromosomes of C. gigas, C. angulata and their hybrids were treated with two different restriction enzymes (ApaI and HaeIII), stained with Giemsa, and examined for banding patterns. These chromosome markers allowed the parental haploid sets to be identified in the hybrids. The analysis of the banded karyotypes of the interspecific hybrids showed that for each chromosome pair, one of the homologues presented a banding pattern consistent with that of C. gigas and the other homologue presented a banding pattern consistent with that of C angulata. These cytogenetic results substantiate the reported interspecific hybridization between C. gigas and C. angulata. In view of these results and taking into account the present expansion of C. gigas aquaculture in southern Europe, the question of the need for preservation of pure C. angulata stocks should be raised as only a few populations remain in the south of Spain and Portugal. Recently, changes in the genetic composition of populations in southern Portugal have indeed been observed, showing that human activities have created contact zones between the two taxa while no natural sympatric zones exist in Europe. (c) 2007 Elsevier B.V. All rights reserved.
Full Text
File Pages Size Access
publication-4959.pdf 10 200 KB Open access
Top of the page

How to cite 

Leitao A, Chaves R, Santos S, Guedes Pinto H, Boudry Pierre (2007). Interspecific hybridization in oysters: Restriction enzyme digestion chromosome banding confirms Crassostrea angulata x Crassostrea gigas F1 hybrids. Journal of Experimental Marine Biology and Ecology, 343(2), 253-260. Publisher's official version : https://doi.org/10.1016/j.jembe.2006.12.007 , Open Access version : https://archimer.ifremer.fr/doc/00000/4959/