Ostreid herpes virus 1 infection in families of the Pacific oyster, Crassostrea gigas, during a summer mortality outbreak: Differences in viral DNA detection and quantification using real-time PCR

Type Article
Date 2009-06
Language English
Author(s) Sauvage Christopher1, Pepin Jean-Francois1, Lapegue SylvieORCID1, Boudry PierreORCID2, Renault Tristan1
Affiliation(s) 1 : IFREMER, Lab Genet & Pathol, F-17390 La Tremblade, France.
2 : IFREMER, UMR Physiol & Ecophysiol Mollusques Marins M100, F-29280 Plouzane, France.
Source Virus Research (0168-1702) (Elsevier), 2009-06 , Vol. 142 , N. 1-2 , P. 181-187
DOI 10.1016/j.virusres.2009.02.013
WOS© Times Cited 89
Keyword(s) Viral DNA quantification, Real time PCR, OsHV 1, Mortality, Crassostrea gigas
Abstract Ostreid herpes virus 1 (OsHV-1) infections, notably reported in Europe and the USA, are closely associated with significant mortalities of the Pacific oyster, Crassostrea gigas, especially during its early stages of life. In summer 2006, we monitored mortality by strict daily verification of three full-sib families of oysters reared under common conditions. We quantified OsHV-1 using real-time PCR in dead and living individuals during and after a mortality event. Mortality events were severe and brief, but significantly different between tested families (cumulative mortality ranging from 1.2 to 49%). Real-time PCR assays revealed different viral DNA loads in dead individuals from different families (P < 0.001). Moreover, the mean level of infection among families was correlated with mortality (P < 0.05). Living oysters showed a significantly lower amount of viral DNA compared with dead ones. This is the first experiment showing the daily changes of individual OsHV-1 DNA load during a mortality outbreak. Our results also support the previously reported high genetic basis underlying the variance of resistance of Pacific oyster to summer mortality, suggesting that there might be a possibility to improve resistance to OsHV-1 by selective breeding.
Full Text
File Pages Size Access
publication-6265.pdf 10 217 KB Open access
Top of the page

How to cite 

Sauvage Christopher, Pepin Jean-Francois, Lapegue Sylvie, Boudry Pierre, Renault Tristan (2009). Ostreid herpes virus 1 infection in families of the Pacific oyster, Crassostrea gigas, during a summer mortality outbreak: Differences in viral DNA detection and quantification using real-time PCR. Virus Research, 142(1-2), 181-187. Publisher's official version : https://doi.org/10.1016/j.virusres.2009.02.013 , Open Access version : https://archimer.ifremer.fr/doc/00000/6265/