Genome sequence of Vibrio splendidus: an abundant planctonic marine species with a large genotypic diversity

Type Article
Date 2009-08
Language English
Author(s) Le Roux Frederique1, 2, Zouine Mohamed1, Chakroun Nesrine1, Binesse Johan1, Saulnier Denis2, Bouchier Christiane3, Zidane Nora3, Ma Laurence3, Rusniok Christophe4, Lajus Aurelie5, Buchrieser Carmen4, Medigue Claudine5, Polz Martin F.6, Mazel Didier1
Affiliation(s) 1 : CNRS, Inst Pasteur, URA 2171, Unite Plast Genome Bacterien, F-75015 Paris, France.
2 : IFREMER, Lab Genet & Pathol, La Tremblade, France.
3 : Inst Pasteur, PF1, Paris, France.
4 : Inst Pasteur, Unite Genom Microorganismes Pathogenes, Paris, France.
5 : CEA, Inst Genom Genoscope & CNRS, UMR8030, Lab Genom Comparat, Evry, France.
6 : MIT, Dept Civil & Environm Engn, Cambridge, MA 02139 USA.
Source Environmental Microbiology (1462-2912) (Wiley / Blackwell), 2009-08 , Vol. 11 , N. 8 , P. 1959-1970
DOI 10.1111/j.1462-2920.2009.01918.x
WOS© Times Cited 77
Abstract P>Vibrio splendidus is a dominant Vibrio species in seawater presenting a remarkable genetic diversity; several strains have been linked to invertebrate's mortality. We report the complete genome sequence of V. splendidus LGP32, an oyster pathogen, and its comparison with partial genome sequences from related strains. As is typical for the genus, V. splendidus LGP32 contains two chromosomes (3.29 and 1.67 Mb) and most essential cellular processes are encoded by chromosome 1. Comparison with two other V. splendidus partial genome sequences (strains 12B01 and Med222) confirms the previously suggested high genotypic diversity within this species and led to the identification of numerous strain-specific regions that could frequently not be assigned to a specific mechanisms of recombination. Surprisingly, the chromosomal integron, the most variable genetic element in all other Vibrio species analysed to date, is absent from 12B01 and inactivated by a mobile element in Med222, while in LGP32 it only contains a limited number of cassettes. Finally, we found that the LGP32 integron contains a new dfrA cassette, related to those found in resistance integrons of Gram-negative clinical isolates. Those results suggest that marine Vibrio can be a source of antibiotic resistance genes.
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Le Roux Frederique, Zouine Mohamed, Chakroun Nesrine, Binesse Johan, Saulnier Denis, Bouchier Christiane, Zidane Nora, Ma Laurence, Rusniok Christophe, Lajus Aurelie, Buchrieser Carmen, Medigue Claudine, Polz Martin F., Mazel Didier (2009). Genome sequence of Vibrio splendidus: an abundant planctonic marine species with a large genotypic diversity. Environmental Microbiology, 11(8), 1959-1970. Publisher's official version : https://doi.org/10.1111/j.1462-2920.2009.01918.x , Open Access version : https://archimer.ifremer.fr/doc/00000/6632/