Combining Two-Stage Testing and Interval Mapping Strategies to Detect QTL for Resistance to Bonamiosis in the European Flat Oyster Ostrea edulis

Type Article
Date 2009-10
Language English
Author(s) Lallias Delphine1, 2, Gomez-Raya L.3, Haley C. S.4, 5, 6, Arzul IsabelleORCID2, Heurtebise Serge2, Beaumont A. R.1, Boudry PierreORCID7, Lapegue Sylvie2
Affiliation(s) 1 : Bangor Univ, Coll Nat Sci, Sch Ocean Sci, Menai Bridge LL59 5AB, Anglesey, Wales.
2 : IFREMER, Lab Genet & Pathol, F-17390 La Tremblade, France.
3 : Univ Nevada, Dept Anim Biotechnol, Reno, NV 89557 USA.
4 : Univ Edinburgh, Roslin Inst, Roslin BioCtr, Roslin EH25 9PS, Midlothian, Scotland.
5 : Univ Edinburgh, Royal Dick Sch Vet Studies, Roslin BioCtr, Roslin EH25 9PS, Midlothian, Scotland.
6 : Western Gen Hosp, MRC Human Genet Unit, Edinburgh EH4 2XU, Midlothian, Scotland.
7 : IFREMER, UMR Physiol & Ecophysiol Mollusques Marins M100, Plouzane, France.
Source Marine Biotechnology (1436-2228) (Springer), 2009-10 , Vol. 11 , N. 5 , P. 570-584
DOI 10.1007/s10126-008-9173-y
WOS© Times Cited 30
Keyword(s) Statistical power, Survival analysis, QTL mapping, Disease resistance, Ostrea edulis
Abstract We have identified quantitative trait loci (QTL) in the flat oyster (Ostrea edulis) for resistance to Bonamia ostreae, a parasite responsible for the dramatic reduction in the aquaculture of this species. An F-2 family from a cross between a wild oyster and an individual from a family selected for resistance to bonamiosis was cultured with wild oysters injected with the parasite, leading to 20% cumulative mortality. Selective genotyping of 92 out of a total of 550 F-2 progeny (i.e., 46 heavily infected oysters that died and 46 parasite-free oysters that survived) was performed using 20 microsatellites and 34 amplification fragment length polymorphism primer pairs. Both a two-stage testing strategy and QTL interval mapping methods were used. The two-stage detection strategy had a high power with a low rate of false positives and identified nine and six probable markers linked to genes of resistance and susceptibility, respectively. Parent-specific genetic linkage maps were built for the family, spanning ten linkage groups (n = 10) with an observed genome coverage of 69-84%. Three QTL were identified by interval mapping in the first parental map and two in the second. Good concordance was observed between the results obtained after the two-stage testing strategy and QTL mapping.
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Lallias Delphine, Gomez-Raya L., Haley C. S., Arzul Isabelle, Heurtebise Serge, Beaumont A. R., Boudry Pierre, Lapegue Sylvie (2009). Combining Two-Stage Testing and Interval Mapping Strategies to Detect QTL for Resistance to Bonamiosis in the European Flat Oyster Ostrea edulis. Marine Biotechnology, 11(5), 570-584. Publisher's official version : https://doi.org/10.1007/s10126-008-9173-y , Open Access version : https://archimer.ifremer.fr/doc/00000/6682/