Combining Two-Stage Testing and Interval Mapping Strategies to Detect QTL for Resistance to Bonamiosis in the European Flat Oyster Ostrea edulis
|Author(s)||Lallias Delphine1, 2, Gomez-Raya L.3, Haley C. S.4, 5, 6, Arzul Isabelle2, Heurtebise Serge2, Beaumont A. R.1, Boudry Pierre7, Lapegue Sylvie2|
|Affiliation(s)||1 : Bangor Univ, Coll Nat Sci, Sch Ocean Sci, Menai Bridge LL59 5AB, Anglesey, Wales.
2 : IFREMER, Lab Genet & Pathol, F-17390 La Tremblade, France.
3 : Univ Nevada, Dept Anim Biotechnol, Reno, NV 89557 USA.
4 : Univ Edinburgh, Roslin Inst, Roslin BioCtr, Roslin EH25 9PS, Midlothian, Scotland.
5 : Univ Edinburgh, Royal Dick Sch Vet Studies, Roslin BioCtr, Roslin EH25 9PS, Midlothian, Scotland.
6 : Western Gen Hosp, MRC Human Genet Unit, Edinburgh EH4 2XU, Midlothian, Scotland.
7 : IFREMER, UMR Physiol & Ecophysiol Mollusques Marins M100, Plouzane, France.
|Source||Marine Biotechnology (1436-2228) (Springer), 2009-10 , Vol. 11 , N. 5 , P. 570-584|
|WOS© Times Cited||33|
|Keyword(s)||Statistical power, Survival analysis, QTL mapping, Disease resistance, Ostrea edulis|
|Abstract||We have identified quantitative trait loci (QTL) in the flat oyster (Ostrea edulis) for resistance to Bonamia ostreae, a parasite responsible for the dramatic reduction in the aquaculture of this species. An F-2 family from a cross between a wild oyster and an individual from a family selected for resistance to bonamiosis was cultured with wild oysters injected with the parasite, leading to 20% cumulative mortality. Selective genotyping of 92 out of a total of 550 F-2 progeny (i.e., 46 heavily infected oysters that died and 46 parasite-free oysters that survived) was performed using 20 microsatellites and 34 amplification fragment length polymorphism primer pairs. Both a two-stage testing strategy and QTL interval mapping methods were used. The two-stage detection strategy had a high power with a low rate of false positives and identified nine and six probable markers linked to genes of resistance and susceptibility, respectively. Parent-specific genetic linkage maps were built for the family, spanning ten linkage groups (n = 10) with an observed genome coverage of 69-84%. Three QTL were identified by interval mapping in the first parental map and two in the second. Good concordance was observed between the results obtained after the two-stage testing strategy and QTL mapping.|