Structure and function of a novel endonuclease acting on branched DNA substrates

Type Article
Date 2009-08
Language English
Author(s) Ren Bin2, Kuehn Joelle3, Meslet-Cladiere Laurence3, Briffotaux Julien4, 5, Norais Cedric3, Lavigne Regis6, Flament DidierORCID4, 5, Ladenstein Rudolf2, Myllykallio Hannu1, 3
Affiliation(s) 1 : Ecole Polytech, INSERM, Lab Opt & Biosci, CNRS,Unite Mixte Rech 7645,U696, F-91128 Palaiseau, France.
2 : Karolinska Inst, Novum, Ctr Struct Biochem, Huddinge, Sweden.
3 : Univ Paris 11, CNRS, Inst Genet & Microbiol, UMR8621, F-91405 Orsay, France.
4 : IFREMER, Lab Microbiol Environm Extremes, UMR 6197, Plouzane, France.
5 : Univ Bretagne Occidentale, Lab Microbiol Environm Extremes, UMR 6197, Plouzane, France.
6 : Univ Rennes 1, INSERM, U625, Rennes, France.
Source Embo Journal (0261-4189) (Nature Publishing Group), 2009-08 , Vol. 28 , N. 16 , P. 2479-2489
DOI 10.1038/emboj.2009.192
WOS© Times Cited 35
Keyword(s) Structure function studies, RecB family, Novel endonuclease, DNA repair, Branched DNA structures
Abstract We show that Pyrococcus abyssi PAB2263 (dubbed NucS (nuclease for ss DNA) is a novel archaeal endonuclease that interacts with the replication clamp PCNA. Structural determination of P. abyssi NucS revealed a two-domain dumbbell-like structure that in overall does not resemble any known protein structure. Biochemical and structural studies indicate that NucS orthologues use a non-catalytic ssDNA-binding domain to regulate the cleavage activity at another site, thus resulting into the specific cleavage at double-stranded DNA (dsDNA)/ssDNA junctions on branched DNA substrates. Both 3' and 5' extremities of the ssDNA can be cleaved at the nuclease channel that is too narrow to accommodate duplex DNA. Altogether, our data suggest that NucS proteins constitute a new family of structure-specific DNA endonucleases that are widely distributed in archaea and in bacteria, including Mycobacterium tuberculosis.
Full Text
File Pages Size Access
publication-6807.pdf 10 331 KB Open access
Top of the page

How to cite 

Ren Bin, Kuehn Joelle, Meslet-Cladiere Laurence, Briffotaux Julien, Norais Cedric, Lavigne Regis, Flament Didier, Ladenstein Rudolf, Myllykallio Hannu (2009). Structure and function of a novel endonuclease acting on branched DNA substrates. Embo Journal, 28(16), 2479-2489. Publisher's official version : , Open Access version :