||Laurent Sebastien1, Colas Florent1, Hamelin Muriel1, Crassous Marie-Pierre1, Antoine Elisabeth1, Lehaitre Michel1, Compere Chantal1
||1 : IFREMER, ERT Interfaces & Capteurs, F-29280 Plouzane, France.
||Sensors Applications Symposium, 2009. SAS 2009. IEEE (IEEE), 2009-02 , P. 29-33
|WOS© Times Cited
||PCR, Algal detection, In situ, Spectroscopy, Surface plasmon resonance
||Among marine algae species, Alexandrium minutum produces a phycotoxin called paralytic shellfish poisoning (PSP) that is introduced in the food chain through the ingestion of phytoplankton by shellfishs, and later by human consumers. Thus, in situ monitoring of A. minutum proliferation in coastal seawater is of great economical importance for marine resources exploitation. Here, we propose a rapid test for the detection of A. minutum by surface plasmon resonance spectroscopy. First, whole genomic DNA is extracted from the algae. Second, a 677 bp long portion of the 28S ribosomal DNA is amplified by PCR. Third, the PCR product is detected by surface plasmon resonance spectroscopy onto a DNA functionalized gold substrate.