Diversity of enterovirus sequences detected in oysters by RT-heminested PCR

Type Article
Date 2004-04
Language English
Author(s) Dubois Eric, Merle Ghislaine, Roquier Catherine, Trompette Aurélien, Le Guyader Soizick, Cruciere Catherine, Chomel Jean-Jacques
Affiliation(s) Agence Francoise Secur Sanitaire Aliments, Secteur Virol Aliments & Eau, F-94703 Maisons Alfort, France.
Univ Lyon 1, Virol Lab, Ctr Natl Reference Enterovirus, F-69373 Lyon 08, France.
IFREMER, Microbiol Lab, F-44311 Nantes 03, France.
Source International Journal of Food Microbiology (0168-1605) (Elsevier), 2004-04 , Vol. 92 , N. 1 , P. 35-43
DOI 10.1016/j.ijfoodmicro.2003.06.001
WOS© Times Cited 16
Keyword(s) Sequence analyze, RT PCR, Cell culture, Shellfish, Enterovirus
Abstract Oysters harvested in western France, from five sites associated with outbreaks of food-borne norovirus gastroenteritis between February 2000 and March 2001, were assayed for enterovirus RNA by reverse transcriptase-heminested polymerase chain reaction (RT-heminested PCR). Forty percent (21/52) of shellfish samples (pool of seven oysters) were contaminated by enteroviruses. Infectious coxsackieviruses serotype A21 were isolated from three of these positive samples. Amplicons corresponding to 65 base sequences in the 5' untranslated region of the enteroviral genome were analyzed by direct sequencing. Interpretable results were obtained from 18 amplicons, but mixtures of sequences confused the results from 3 samples. Sequences isolated from samples from the different sites were different but similarities were observed between sequences detected in shellfish from two sites at different dates. Sequences were also compared to sequences of human, bovine and porcine enteroviruses. Both human and animal origins of enterovirus contamination of shellfish seemed likely.
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