Type |
Article |
Date |
2005-06 |
Language |
English |
Author(s) |
Le Roux Frederique1, Goubet A1, Thompson F2, Faury Nicole1, Gay Melanie1, Swings J2, Saulnier Denis1 |
Affiliation(s) |
1 : IFREMER, Lab Genet & Pathol, F-17390 La Tremblade, France. 2 : State Univ Ghent, Lab Microbiol & BCCM, LMG Bacteria Collect, Microbiol Lab, B-9000 Ghent, Belgium. |
Source |
International journal of systematic and evolutionary microbiology (1466-5026) (Society for General Microbiology), 2005-06 , Vol. 55 , N. 6 , P. 2251-2255 |
DOI |
10.1099/ijs.0.63666-0 |
WOS© Times Cited |
58 |
Keyword(s) |
Vibrio gigantis, Haemoplymph, Disease, Crassostrea gigas, Oyster culture |
Abstract |
Polyphasic analysis of four new Vibrio isolates originating from the haemolymph of diseased cultured oysters is described. The new isolates were closely related to Vibrio splendidus, having 98 % 16S rRNA gene sequence similarity. Phylogenetic analysis based on DNA gyrase subunit B (gyrB), RNA polymerase Quo factor (rpoD), replication origin-binding protein (rctB) and transmembrane regulatory protein (toxR) genes, fluorescent amplified fragment length polymorphism and DNA-DNA hybridization experiments clearly showed that the new isolates form a tight genomic group that is different from the currently known Vibrio species. It is proposed that these new isolates should be accommodated in a novel species, Vibrio gigantis sp. nov. Phenotypic features that differentiate V. gigantis from other known Vibrio species include arginine dihydrolase, gelatinase and beta-galactosidase activities, NO2 production, growth at 35 degrees C, and utilization of sucrose, melibiose, amygdalin, glycerol, galactose, starch and glycogen. The type strain is LGP 13(T) (=LMG 22741(T)=CIP 108656(T)). |
Full Text |
File |
Pages |
Size |
Access |
publication-792.pdf |
5 |
123 KB |
Open access |
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