||Li Ronghua1, 2, Li Qi2, Cornette Florence1, Degremont Lionel1, Lapegue Sylvie1
||1 : IFREMER, Lab Genet & Pathol, F-17390 La Tremblade, France.
2 : Ocean Univ China, Coll Fisheries, Qingdao 266003, Peoples R China.
||Aquaculture (0044-8486) (Elsevier Science Bv), 2010-12 , Vol. 310 , N. 1-2 , P. 234-239
|WOS© Times Cited
||Pacific oyster, Crassostrea gigas, Microsatellite, EST, Multiplex PCR, Parentage assignment
||We report four highly informative multiplex PCRs developed from twelve previously described ESTSSRs in Crassostrea gigas. We evaluated and validated these multiplex PCRs in twelve full-sib families. The average allelic richness and the polymorphism information content (PIC) were 11.1 and 0.811 respectively. The combined power of exclusion was greater than 99.99% using all four multiplex assays. A hundred and forty three tests of segregation ratios revealed 11 significant departures from expected Mendelian ratios. The frequency of null alleles was estimated as 4.9% of all the alleles segregating based on a within-family analysis of Mendelian segregation patterns. Parentage analysis of real offspring demonstrated that 97% of all offspring were unambiguously allocated to a pair of parents based on two multiplex PCRs with only a 4% error rate, and 100% of the offspring were correctly allocated to their parents when three multiplex PCRs were used.
|Author's final draft
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