| Type |
Article |
| Date |
2010-12 |
| Language |
English |
| Author(s) |
Li Ronghua1, 2, Li Qi2, Cornette Florence1, Degremont Lionel 1, Lapegue Sylvie1 |
| Affiliation(s) |
1 : IFREMER, Lab Genet & Pathol, F-17390 La Tremblade, France.
2 : Ocean Univ China, Coll Fisheries, Qingdao 266003, Peoples R China. |
| Source |
Aquaculture (0044-8486) (Elsevier Science Bv), 2010-12 , Vol. 310 , N. 1-2 , P. 234-239 |
| DOI |
10.1016/j.aquaculture.2010.09.037 |
| WOS© Times Cited |
25 |
| Keyword(s) |
Pacific oyster, Crassostrea gigas, Microsatellite, EST, Multiplex PCR, Parentage assignment |
| Abstract |
We report four highly informative multiplex PCRs developed from twelve previously described ESTSSRs in Crassostrea gigas. We evaluated and validated these multiplex PCRs in twelve full-sib families. The average allelic richness and the polymorphism information content (PIC) were 11.1 and 0.811 respectively. The combined power of exclusion was greater than 99.99% using all four multiplex assays. A hundred and forty three tests of segregation ratios revealed 11 significant departures from expected Mendelian ratios. The frequency of null alleles was estimated as 4.9% of all the alleles segregating based on a within-family analysis of Mendelian segregation patterns. Parentage analysis of real offspring demonstrated that 97% of all offspring were unambiguously allocated to a pair of parents based on two multiplex PCRs with only a 4% error rate, and 100% of the offspring were correctly allocated to their parents when three multiplex PCRs were used. |
| Full Text |
| File |
Pages |
Size |
Access |
| Author's final draft |
13 |
114 KB |
Open access |
|
6 |
235 KB |
Access on demand |
|
