Mussel aquaculture is well developed in various ecosystems of temperate waters. In the aim of developing predictive tools for management of mussel aquaculture, a better understanding of relationships between the environmental conditions and mussel growth is necessary. For this purpose, development of bioenergetic models, linking environmental variables (especially food resource and temperature) and mussel growth and reproduction, are of a particular interest. Dynamic Energy Budget (DEB) theory offers a general framework to study energy flows in organisms, from assimilation to use for maintenance, growth and reproduction. This theory is appropriate for growth modelling of marine organisms and have first been applied in 1993 to model mussel growth. Nevertheless, the different studies that applied DEB theory on the mussel Mytilus edulis lead to different parameter values though based on the same equations. In this context, our work aims to refine parameters estimation of the DEB model for M. edulis in order to obtain a generic parameter set, i.e. which allows modelling mussel growth and reproduction in various environments. For this purpose, methods which have been developed recently for parameter estimation have been applied using data from the literature. New parameter estimates are compared to previously obtained values and are validated by simulating mussel growth and reproduction in various environments.