Detection of Type 1 Ostreid Herpes variant (OsHV-1 mu var) with no associated mortality in French-origin Pacific cupped oyster Crassostrea gigas farmed in Italy
|Author(s)||Dundon William G.1, Arzul Isabelle2, Omnes Emmanuelle2, Robert Maeva2, Magnabosco Cristian3, Zambon Michela3, Gennari Lorenzo, Toffan Anna1, Terregino Calogero1, Capua Ilaria1, Arcangeli Giuseppe3|
|Affiliation(s)||1 : Ist Zooprofilatt Sperimentale Venezie, Dept Res & Dev, I-35020 Legnaro, PD, Italy.
2 : IFREMER, Lab Genet & Pathol, La Tremblade, France.
3 : Ist Zooprofilatt Sperimentale Venezie, Natl Reference Ctr Fish Molluscs & Crustaceans Di, Adria, RO, Italy.
|Source||Aquaculture (0044-8486) (Elsevier Science Bv), 2011-04 , Vol. 314 , N. 1-4 , P. 49-52|
|WOS© Times Cited||62|
|Keyword(s)||Pacific cupped oyster, Ostreid Herpes virus 1 mu var, PCR, Italy|
|Abstract||In April 2010, the sampling of juvenile Pacific cupped oysters originating from France was undertaken from a farm located off the coast of the Marche region in Italy. Samples were sent to the national reference laboratory for mollusc diseases for bacteriological, histological, electron microscopical and molecular analysis. Classical and Real-Time PCR indicated the presence of the microvariant (OsHV-1 mu var) of Type 1 Ostreid Herpes virus (OsHV-1) despite the absence of clinical and pathological signs normally associated with the presence of this variant. Further molecular and sequencing analysis revealed the presence of OsHV-1 par and OsHV-1 DNA in one oyster indicating that simultaneous detection of both viruses is possible. This is the first report of the presence of OsHV-1 mu var in Italy and also the first time that evidence of possible co-infection of OsHV-1 and OsHV-1 mu var has been provided. (C) 2011 Elsevier B.V. All rights reserved.|