We present here the first mercury speciation study in the water column of the Southern Ocean, using a high-resolution south-to-north section (27 stations from 65.50 degrees S to 44.00 degrees S) with up to 15 depths (0-4440 m) between Antarctica and Tasmania (Australia) along the 140 degrees E meridian. In addition, in order to explore the role of sea ice in Hg cycling, a study of mercury speciation in the "snow-sea ice-seawater" continuum was conducted at a coastal site, near the Australian Casey station (66.40 degrees S; 101.14 degrees E). In the open ocean waters, total Hg (Hg(T)) concentrations varied from 0.63 to 2.76 pmol L(-1) with "transient-type" vertical profiles and a latitudinal distribution suggesting an atmospheric mercury source south of the Southern Polar Front (SPF) and a surface removal north of the Subantartic Front (SAF). Slightly higher mean Hg(T) concentrations (1.35 +/- 0.39 pmol L(-1)) were measured in Antarctic Bottom Water (AABW) compared to Antarctic Intermediate water (AAIW) (1.15 +/- 0.22 pmol L(-1)). Labile Hg (Hg(R)) concentrations varied from 0.01 to 2.28 pmol L(-1), with a distribution showing that the Hg(T) enrichment south of the SPF consisted mainly of Hg(R) (67 +/- 23%), whereas, in contrast, the percentage was half that in surface waters north of PFZ (33 +/- 23%). Methylated mercury species (MeHg(T)) concentrations ranged from 0.02 to 0.86 pmol L(-1). All vertical MeHg(T) profiles exhibited roughly the same pattern, with low concentrations observed in the surface layer and increasing concentrations with depth up to an intermediate depth maximum. As for Hg(T), low mean MeHg(T) concentrations were associated with AAIW, and higher ones with AABW. The maximum of MeHg(T) concentration at each station was systematically observed within the oxygen minimum zone, with a statistically significant MeHg(T) vs Apparent Oxygen Utilization (AOU) relationship (p < 0.001). The proportion of Hg(T) as methylated species was lower than 5% in the surface waters, around 50% in deep waters below 1000 m, reaching a maximum of 78% south of the SPF. At Casey coastal station Hg(T) and Hg(R) concentrations found in the "snow-sea ice-seawater" continuum were one order of magnitude higher than those measured in open ocean waters. The distribution of Hg(T) there suggests an atmospheric Hg deposition with snow and a fractionation process during sea ice formation, which excludes Hg from the ice with a parallel Hg enrichment of brine, probably concurring with the Hg enrichment of AABW observed in the open ocean waters. Contrastingly, MeHg(T) concentrations in the sea ice environment were in the same range as in the open ocean waters, remaining below 0.45 pmol L(-1). The MeHg(T) vertical profile through the continuum suggests different sources, including atmosphere, seawater and methylation in basal ice. Whereas Hg(T) concentrations in the water samples collected between the Antarctic continent and Tasmania are comparable to recent measurements made in the other parts of the World Ocean (e. g., Soerensen et al., 2010), the Hg species distribution suggests distinct features in the Southern Ocean Hg cycle: (i) a net atmospheric Hg deposition on surface water near the ice edge, (ii) the Hg enrichment in brine during sea ice formation, and (iii) a net methylation of Hg south of the SPF. (C) 2011 Elsevier Ltd. All rights resrved.