Persistence of microbial and chemical pig manure markers as compared to faecal indicator bacteria survival in freshwater and seawater microcosms

Type Article
Date 2011-10
Language English
Author(s) Solecki O.1, 2, Jeanneau L.3, Jarde E.3, Gourmelon MicheleORCID4, Marin Charlotte4, Pourcher A. M.1, 2
Affiliation(s) 1 : Cemagref, F-35044 Rennes, France
2 : Univ Europeenne Bretagne, Rennes, France
3 : CNRS, UMR 6118 Geosci Rennes, Rennes, France
4 : IFREMER, EMP, Microbiol Lab, Plouzane, France
Source Water Research (0043-1354) (Pergamon-elsevier Science Ltd), 2011-10 , Vol. 45 , N. 15 , P. 4623-4633
DOI 10.1016/j.watres.2011.06.012
WOS© Times Cited 32
Keyword(s) Faecal source tracking, Decay rates, Faecal stanols, Biphasic kinetic, Lactobacillus amylovorus, Bacteroidales marker
Abstract Natural seawater and freshwater microcosms inoculated with pig manure were set up to determine the persistence of pig faecal microbial and chemical markers in these two types of surface water. The concentrations of Lactobacillus amylovorus, the Bacteroidales Pig-2-Bac 165 rRNA genetic marker, five stanols and the evolution of two ratios of stanols, R(1) (coprostanol to the sum of coprostanol and 24-ethylcoprostanol) and R(2) (sitostanol to coprostanol) were analyzed during two months along with the concentration of Faecal Indicator Bacteria (FIB). Pig manure was inoculated to unfiltered water microcosms incubated aerobically at 18 degrees C in the dark. The faecal contamination load represented by the concentrations of culturable Escherichia coli and/or enterococci remained for two months in the freshwater and seawater microcosms water column. These concentrations followed a biphasic decay pattern with a 97% reduction of the initial amount during a first rapid phase (<6 days) and a remaining proportion undergoing a slower or null second decline. The L. amylovorus marker and five stanols persisted as long as the indicators in both treatments. The Pig-2-Bac marker persisted 20 and 27 days in seawater and freshwater, respectively. The ratios R(1) and R(2) were in the range specific to pig manure until day 6 in both types of water. These results indicate that Pig-2-Bac, L. amylovorus and stanol ratios might be used in combination to complement FIB testing to determine the pig source of fecal pollution. However, stanol ratios are to be used when the time point of the discharge is known. (C) 2011 Elsevier Ltd. All rights reserved.
Full Text
File Pages Size Access
11 569 KB Access on demand
Author's final draft 31 448 KB Open access
Top of the page