Development of a Mass Sensitive Quartz Crystal Microbalance (QCM)-Based DNA Biosensor Using a 50 MHz Electronic Oscillator Circuit
| Type | Article | ||||||||
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| Date | 2011-08 | ||||||||
| Language | English | ||||||||
| Author(s) | Garcia-Martinez Gonzalo1, Alonso Bustabad Enrique1, Perrot Hubert2, 3, Gabrielli Claude2, Bucur Bogdan2, 3, Lazerges Mathieu2, 3, Rose Daniel2, 3, Rodriguez-Pardo Loreto1, Farina Jose1, Compere Chantal 4, Arnau Vives Antonio5 |
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| Affiliation(s) | 1 : CNRS, LISE, UPR15, F-75252 Paris 05, France 2 : Univ Vigo, Dpto Tecnol Elect, Vigo 36310, Spain 3 : Univ Paris 06, F-75252 Paris 05, France 4 : IFREMER, Ctr Brest, Serv Interfaces & Capteurs, F-29280 Plouzane, France 5 : Univ Politecn Valencia, Wave Phenomena Grp, Dept Elect Engn, Valencia 46022, Spain |
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| Source | Sensors (1424-8220) (Mdpi Ag), 2011-08 , Vol. 11 , N. 8 , P. 7656-7664 | ||||||||
| DOI | 10.3390/s110807656 | ||||||||
| WOS© Times Cited | 43 | ||||||||
| Keyword(s) | DNA sequence detection, quartz crystal microbalance, electronic oscillator, sensitivity, frequency noise, resolution | ||||||||
| Abstract | This work deals with the design of a high sensitivity DNA sequence detector using a 50 MHz quartz crystal microbalance (QCM) electronic oscillator circuit. The oscillator circuitry is based on Miller topology, which is able to work in damping media. Calibration and experimental study of frequency noise are carried out, finding that the designed sensor has a resolution of 7.1 ng/cm(2) in dynamic conditions (with circulation of liquid). Then the oscillator is proved as DNA biosensor. Results show that the system is able to detect the presence of complementary target DNAs in a solution with high selectivity and sensitivity. DNA target concentrations higher of 50 ng/mL can be detected. | ||||||||
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