Effect of oxygen and temperature on the dynamic of the dominant bacterial populations of pig manure and on the persistence of pig-associated genetic markers, assessed in river water microcosms
| Type | Article | ||||||||||||
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| Date | 2011-11 | ||||||||||||
| Language | English | ||||||||||||
| Author(s) | Marti R.1, 2, Mieszkin Sophie3, Solecki O.1, 2, Pourcher A. -M.1, 2, Hervio-Heath Dominique3, Gourmelon Michele 3 |
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| Affiliation(s) | 1 : Cemagref, UR GERE, Rennes, France. 2 : Univ Europeenne Bretagne, Rennes, France. 3 : IFREMER, EMP, Lab Microbiol, F-29280 Plouzane, France. |
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| Source | Journal Of Applied Microbiology (1364-5072) (Wiley-blackwell), 2011-11 , Vol. 111 , N. 5 , P. 1159-1175 | ||||||||||||
| DOI | 10.1111/j.1365-2672.2011.05131.x | ||||||||||||
| WOS© Times Cited | 15 | ||||||||||||
| Keyword(s) | Lactobacillus amylovorus, microbial source tracking, oxygen, persistence, pig-associated Bacteroidales markers, quantitative real-time PCR, river water, temperature | ||||||||||||
| Abstract | Aims: The aim is to evaluate the dynamic of Bacteroides-Prevotella and Bacillus-Streptococcus-Lactobacillus populations originating from pig manure and the persistence of pig-associated markers belonging to these groups according to temperature and oxygen. Methods and Results: River water was inoculated with pig manure and incubated under microaerophilic and aerobic conditions, at 4 and 20 degrees C over 43 days. The diversity of bacterial populations was analysed by capillary electrophoresis-single-strand conformation polymorphism. The persistence of the pig-associated markers was measured by real-time PCR and compared with the survival of Escherichia coli and enterococci. Decay was characterized by the estimation of the time needed to produce a 1-log reduction (T90). The greatest changes were observed at 20 degrees C under aerobic conditions, leading to a reduction in the diversity of the bacterial populations and in the concentrations of the Pig-1-Bac, Pig-2-Bac and Lactobacillus amylovorus markers with a T90 of 10 5, 8 1 and 17 2 days, respectively. Conclusions: Oxygen and temperature were found to have a combined effect on the persistence of the pig-associated markers in river waters. Significance and Impact of the Study: The persistence profiles of the Pig-1-Bac, Pig-2-Bac and Lact. amylovorus markers in addition to their high specificity and sensitivity support their use as relevant markers to identify pig faecal contamination in river waters. | ||||||||||||
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