Effect of oxygen and temperature on the dynamic of the dominant bacterial populations of pig manure and on the persistence of pig-associated genetic markers, assessed in river water microcosms

Type Article
Date 2011-11
Language English
Author(s) Marti R.1, 2, Mieszkin Sophie3, Solecki O.1, 2, Pourcher A. -M.1, 2, Hervio-Heath Dominique3, Gourmelon MicheleORCID3
Affiliation(s) 1 : Cemagref, UR GERE, Rennes, France.
2 : Univ Europeenne Bretagne, Rennes, France.
3 : IFREMER, EMP, Lab Microbiol, F-29280 Plouzane, France.
Source Journal Of Applied Microbiology (1364-5072) (Wiley-blackwell), 2011-11 , Vol. 111 , N. 5 , P. 1159-1175
DOI 10.1111/j.1365-2672.2011.05131.x
WOS© Times Cited 14
Keyword(s) Lactobacillus amylovorus, microbial source tracking, oxygen, persistence, pig-associated Bacteroidales markers, quantitative real-time PCR, river water, temperature
Abstract Aims: The aim is to evaluate the dynamic of Bacteroides-Prevotella and Bacillus-Streptococcus-Lactobacillus populations originating from pig manure and the persistence of pig-associated markers belonging to these groups according to temperature and oxygen. Methods and Results: River water was inoculated with pig manure and incubated under microaerophilic and aerobic conditions, at 4 and 20 degrees C over 43 days. The diversity of bacterial populations was analysed by capillary electrophoresis-single-strand conformation polymorphism. The persistence of the pig-associated markers was measured by real-time PCR and compared with the survival of Escherichia coli and enterococci. Decay was characterized by the estimation of the time needed to produce a 1-log reduction (T90). The greatest changes were observed at 20 degrees C under aerobic conditions, leading to a reduction in the diversity of the bacterial populations and in the concentrations of the Pig-1-Bac, Pig-2-Bac and Lactobacillus amylovorus markers with a T90 of 10 5, 8 1 and 17 2 days, respectively. Conclusions: Oxygen and temperature were found to have a combined effect on the persistence of the pig-associated markers in river waters. Significance and Impact of the Study: The persistence profiles of the Pig-1-Bac, Pig-2-Bac and Lact. amylovorus markers in addition to their high specificity and sensitivity support their use as relevant markers to identify pig faecal contamination in river waters.
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Marti R., Mieszkin Sophie, Solecki O., Pourcher A. -M., Hervio-Heath Dominique, Gourmelon Michele (2011). Effect of oxygen and temperature on the dynamic of the dominant bacterial populations of pig manure and on the persistence of pig-associated genetic markers, assessed in river water microcosms. Journal Of Applied Microbiology, 111(5), 1159-1175. Publisher's official version : https://doi.org/10.1111/j.1365-2672.2011.05131.x , Open Access version : https://archimer.ifremer.fr/doc/00053/16470/