SSR-based analysis of clonality, spatial genetic structure and introgression from the Lombardy poplar into a natural population of Populus nigra L. along the Loire River

Type Article
Date 2011-12
Language English
Author(s) Chenault Nicolas1, Arnaud-Haond SophieORCID2, Juteau Mary1, Valade Romain1, Almeida Jose-Luis3, Villar Marc1, Bastien Catherine1, Dowkiw Arnaud1
Affiliation(s) 1 : INRA, UR Ameliorat Genet & Physiol Forestieres 0588, CS Ardon 40001, F-45075 Orleans 2, France.
2 : IFREMER, Lab Environm Profond, Ctr Brest, F-29280 Plouzane, France.
3 : INRA, UE Genet & Biomasse Forestieres 0995, CS Ardon 40001, F-45075 Orleans 2, France.
Source Tree Genetics & Genomes (1614-2942) (Springer Heidelberg), 2011-12 , Vol. 7 , N. 6 , P. 1249-1262
DOI 10.1007/s11295-011-0410-6
WOS© Times Cited 27
Keyword(s) Populus nigra, Lombardy poplar, Clonality, Spatial genetic structure, Introgression, Clonal growth form
Abstract A scarcity of favourable habitats and introgression from exotic cultivars are two major threats to black poplars (Populus nigra L.) in Europe. Natural vegetative propagation contributes to maintenance of the species in areas where seedling recruitment is limited. Exhaustive sampling of all mature trees in a natural P. nigra stand (413 individuals at recorded positions), genotyping at 11 SSR loci, and a standardized analysis framework resulted in a precise description of clonality in terms of (a) frequency, (b) spatial growth form, and (c) impacts on the overall spatial genetic structure (SGS). The high proportion of replicated genotypes detected resulted in a genotypic richness (R) of 0.47. Up to 18 ramets were found per multilocus lineage (MLL), but 95% of MLLs contained fewer than five ramets (Pareto index beta=1.07). No significant difference in vegetative propagation potential was found between genders. Uneven spatial distribution of ramets, with clustering of clonal ramets (aggregation index A(c)=0.62) and near-zero intermingling between MLLs (clonal dominance index D(c)=0.99), resulted in a 'phalanx' clonal growth form, explaining most of the SGS observed over short distances (0-20 m, Sp=0.0324). Although they did not exhibit the typical columnar shape of the Lombardy poplar (P. nigra var. italica), five trees were found to be probable F(1) hybrids of this old and widely distributed cultivar.
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Chenault Nicolas, Arnaud-Haond Sophie, Juteau Mary, Valade Romain, Almeida Jose-Luis, Villar Marc, Bastien Catherine, Dowkiw Arnaud (2011). SSR-based analysis of clonality, spatial genetic structure and introgression from the Lombardy poplar into a natural population of Populus nigra L. along the Loire River. Tree Genetics & Genomes, 7(6), 1249-1262. Publisher's official version : https://doi.org/10.1007/s11295-011-0410-6 , Open Access version : https://archimer.ifremer.fr/doc/00065/17617/