Acquisition of epibiotic bacteria along the life cycle of the hydrothermal shrimp Rimicaris exoculata

Type Article
Date 2012-03
Language English
Author(s) Guri Mathieu2, Durand Lucile1, Cueff-Gauchard Valerie1, Zbinden Magali3, Crassous Philippe1, Shillito Bruce3, Cambon-Bonavita Marie-Anne1
Affiliation(s) 1 : IFREMER, Ctr Brest, Lab Microbiol Environm Extremes, DEEP,UMR6197, F-29280 Plouzane, France.
2 : CNRS, LM2E, UMR6197, Plouzane, France.
3 : Univ Paris 06, CNRS, UMR Systemat Adaptat & Evolut 7138, Paris, France.
Source The ISME journal (Multidisciplinary Journal of Microbial Ecology) (1751-7362) (Nature), 2012-03 , Vol. 6 , N. 3 , P. 597-609
DOI 10.1038/ismej.2011.133
WOS© Times Cited 29
Keyword(s) symbiosis, larvae, methanotrophic symbiont, Rimicaris exoculata, transmission pathways
Abstract The caridean shrimp Rimicaris exoculata dominates the fauna at several Mid-Atlantic Ridge hydrothermal vent sites. This shrimp has an enlarged gill chamber, harboring a dense ectosymbiotic community of chemoautotrophic bacteria associated with mineral oxide deposits. Until now, their acquisition is not fully understood. At three hydrothermal vent sites, we analyzed the epibionts diversity at different moult stages and also in the first stages of the shrimp life (eggs, hatched eggs (with larvae) and juveniles). Hatched eggs associated with young larvae were collected for the first time directly from gravid females at the Logachev vent site during the Serpentine cruise. An approach using 16S rRNA clone libraries, scanning and transmission electron microscopy, and fluorescent in situ hybridization was used. Molecular results and microscope observations indicated a switch in the composition of the bacterial community between early R. exoculata life cycle stage (egg libraries dominated by the Gammaproteobacteria) and later stages (juvenile/adult libraries dominated by the Epsilonproteobacteria). We hypothesized that the epibiotic phylotype composition could vary according to the life stage of the shrimp. Our results confirmed the occurrence of a symbiosis with Gammaproteobacteria and Epsilonproteobacteria, but more complex than previously assumed. We revealed the presence of active type-I methanotrophic bacteria colonizing the cephalothorax of shrimps from the Rainbow site. They were also present on the eggs from the Logachev site. This could be the first 'epibiotic' association between methanotrophic bacteria and hydrothermal vent crustacean. We discuss possible transmission pathways for epibionts linked to the shrimp life cycle. The ISME Journal (2012) 6, 597-609; doi: 10.1038/ismej.2011.133; published online 13 October 2011
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Guri Mathieu, Durand Lucile, Cueff-Gauchard Valerie, Zbinden Magali, Crassous Philippe, Shillito Bruce, Cambon-Bonavita Marie-Anne (2012). Acquisition of epibiotic bacteria along the life cycle of the hydrothermal shrimp Rimicaris exoculata. The ISME journal (Multidisciplinary Journal of Microbial Ecology), 6(3), 597-609. Publisher's official version : https://doi.org/10.1038/ismej.2011.133 , Open Access version : https://archimer.ifremer.fr/doc/00072/18318/