Enhancement of neutral lipid productivity in the microalga Isochrysis affinis Galbana (T-Iso) by a mutation-selection procedure

Microalgae offer a high potential for energetic lipid storage as well as high growth rates. They are therefore considered promising candidates for biofuel production, with the selection of high lipid-producing strains a major objective in projects on the development of this technology. We developed a mutation-selection method aimed at increasing microalgae neutral lipid productivity. A two step method, based on UVc irradiation followed by flow cytometry selection, was applied to a set of strains that had an initial high lipid content and improvement was assessed by means of Nile-red fluorescence measurements. The method was first tested on Isochrysis affinis galbana (T-Iso). (...)

Keyword(s)

strain selection, Isochrysis, neutral lipid, ultra violet, flux cytometry, biofuel

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Bougaran Gael, Rouxel Catherine, Dubois Nolwenn, Kaas Raymond, Grouas Sophie, Lukomska Ewa, Le Coz Jean-Rene, Cadoret Jean-Paul (2012). Enhancement of neutral lipid productivity in the microalga Isochrysis affinis Galbana (T-Iso) by a mutation-selection procedure. Biotechnology And Bioengineering. 109 (11). 2737-2745. https://doi.org/10.1002/bit.24560, https://archimer.ifremer.fr/doc/00085/19643/

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