Type |
Article |
Date |
2012-11 |
Language |
English |
Author(s) |
Bougaran Gael 1, Rouxel Catherine1, Dubois Nolwenn1, Kaas Raymond1, Grouas Sophie1, Lukomska Ewa1, Le Coz Jean-Rene2, Cadoret Jean-Paul1 |
Affiliation(s) |
1 : IFREMER, PBA, F-44311 Nantes 03, France. 2 : IFREMER, LPI, UMR100, PE2M, F-29280 Plouzane, France. |
Source |
Biotechnology And Bioengineering (0006-3592) (Wiley-blackwell), 2012-11 , Vol. 109 , N. 11 , P. 2737-2745 |
DOI |
10.1002/bit.24560 |
WOS© Times Cited |
51 |
Keyword(s) |
strain selection, Isochrysis, neutral lipid, ultra violet, flux cytometry, biofuel |
Abstract |
Microalgae offer a high potential for energetic lipid storage as well as high growth rates. They are therefore considered promising candidates for biofuel production, with the selection of high lipid-producing strains a major objective in projects on the development of this technology. We developed a mutation-selection method aimed at increasing microalgae neutral lipid productivity. A two step method, based on UVc irradiation followed by flow cytometry selection, was applied to a set of strains that had an initial high lipid content and improvement was assessed by means of Nile-red fluorescence measurements. The method was first tested on Isochrysis affinis galbana (T-Iso). (...) |
Full Text |
File |
Pages |
Size |
Access |
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9 |
159 KB |
Access on demand |
Author's final draft |
30 |
141 KB |
Open access |
Correction in press - 10.1002/bit.28294 |
1 |
88 KB |
Open access |
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