Enhancement of neutral lipid productivity in the microalga Isochrysis affinis Galbana (T-Iso) by a mutation-selection procedure

Type Article
Date 2012-11
Language English
Author(s) Bougaran GaelORCID1, Rouxel Catherine1, Dubois Nolwenn1, Kaas Raymond1, Grouas Sophie1, Lukomska Ewa1, Le Coz Jean-Rene2, Cadoret Jean-Paul1
Affiliation(s) 1 : IFREMER, PBA, F-44311 Nantes 03, France.
2 : IFREMER, LPI, UMR100, PE2M, F-29280 Plouzane, France.
Source Biotechnology And Bioengineering (0006-3592) (Wiley-blackwell), 2012-11 , Vol. 109 , N. 11 , P. 2737-2745
DOI 10.1002/bit.24560
WOS© Times Cited 45
Keyword(s) strain selection, Isochrysis, neutral lipid, ultra violet, flux cytometry, biofuel
Abstract Microalgae offer a high potential for energetic lipid storage as well as high growth rates. They are therefore considered promising candidates for biofuel production, with the selection of high lipid-producing strains a major objective in projects on the development of this technology. We developed a mutation-selection method aimed at increasing microalgae neutral lipid productivity. A two step method, based on UVc irradiation followed by flow cytometry selection, was applied to a set of strains that had an initial high lipid content and improvement was assessed by means of Nile-red fluorescence measurements. The method was first tested on Isochrysis affinis galbana (T-Iso). (...)
Full Text
File Pages Size Access
9 159 KB Access on demand
Author's final draft 30 141 KB Open access
Top of the page

How to cite 

Bougaran Gael, Rouxel Catherine, Dubois Nolwenn, Kaas Raymond, Grouas Sophie, Lukomska Ewa, Le Coz Jean-Rene, Cadoret Jean-Paul (2012). Enhancement of neutral lipid productivity in the microalga Isochrysis affinis Galbana (T-Iso) by a mutation-selection procedure. Biotechnology And Bioengineering, 109(11), 2737-2745. Publisher's official version : https://doi.org/10.1002/bit.24560 , Open Access version : https://archimer.ifremer.fr/doc/00085/19643/