Enhancement of neutral lipid productivity in the microalga Isochrysis affinis Galbana (T-Iso) by a mutation-selection procedure

Type Article
Date 2012-11
Language English
Author(s) Bougaran GaelORCID1, Rouxel Catherine1, Dubois Nolwenn1, Kaas Raymond1, Grouas Sophie1, Lukomska Ewa1, Le Coz Jean-Rene2, Cadoret Jean-Paul1
Affiliation(s) 1 : IFREMER, PBA, F-44311 Nantes 03, France.
2 : IFREMER, LPI, UMR100, PE2M, F-29280 Plouzane, France.
Source Biotechnology And Bioengineering (0006-3592) (Wiley-blackwell), 2012-11 , Vol. 109 , N. 11 , P. 2737-2745
DOI 10.1002/bit.24560
WOS© Times Cited 55
Keyword(s) strain selection, Isochrysis, neutral lipid, ultra violet, flux cytometry, biofuel
Abstract Microalgae offer a high potential for energetic lipid storage as well as high growth rates. They are therefore considered promising candidates for biofuel production, with the selection of high lipid-producing strains a major objective in projects on the development of this technology. We developed a mutation-selection method aimed at increasing microalgae neutral lipid productivity. A two step method, based on UVc irradiation followed by flow cytometry selection, was applied to a set of strains that had an initial high lipid content and improvement was assessed by means of Nile-red fluorescence measurements. The method was first tested on Isochrysis affinis galbana (T-Iso). (...)
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Bougaran Gael, Rouxel Catherine, Dubois Nolwenn, Kaas Raymond, Grouas Sophie, Lukomska Ewa, Le Coz Jean-Rene, Cadoret Jean-Paul (2012). Enhancement of neutral lipid productivity in the microalga Isochrysis affinis Galbana (T-Iso) by a mutation-selection procedure. Biotechnology And Bioengineering, 109(11), 2737-2745. Publisher's official version : https://doi.org/10.1002/bit.24560 , Open Access version : https://archimer.ifremer.fr/doc/00085/19643/