||Bougaran Gael1, Rouxel Catherine1, Dubois Nolwenn1, Kaas Raymond1, Grouas Sophie1, Lukomska Ewa1, Le Coz Jean-Rene2, Cadoret Jean-Paul1
||1 : IFREMER, PBA, F-44311 Nantes 03, France.
2 : IFREMER, LPI, UMR100, PE2M, F-29280 Plouzane, France.
||Biotechnology And Bioengineering (0006-3592) (Wiley-blackwell), 2012-11 , Vol. 109 , N. 11 , P. 2737-2745
|WOS© Times Cited
||strain selection, Isochrysis, neutral lipid, ultra violet, flux cytometry, biofuel
||Microalgae offer a high potential for energetic lipid storage as well as high growth rates. They are therefore considered promising candidates for biofuel production, with the selection of high lipid-producing strains a major objective in projects on the development of this technology. We developed a mutation-selection method aimed at increasing microalgae neutral lipid productivity. A two step method, based on UVc irradiation followed by flow cytometry selection, was applied to a set of strains that had an initial high lipid content and improvement was assessed by means of Nile-red fluorescence measurements. The method was first tested on Isochrysis affinis galbana (T-Iso). (...)
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|Author's final draft