Comparative study of three analysis methods (TTGE, flow cytometry and HPLC) for xenobiotic impact assessment on phytoplankton communities
|Author(s)||Stachowski-Haberkorn Sabine1, Quiniou Louis2, Beker Beatriz3, Haberkorn Hansy2, Marie Dominique4, 5, de La Broise Denis1|
|Affiliation(s)||1 : Univ Europeenne Bretagne, LUBEM EA3882, UBO, F-29334 Quimper, France.
2 : Univ Europeenne Bretagne, LEMAR, UMR 6539, UBO,IUEM, F-29280 Plouzane, France.
3 : Ctr Oceanol Marseille, F-13007 Marseille, France.
4 : CNRS, Stn Biol Roscoff, F-29682 Roscoff, France.
5 : Univ Paris 06, F-29682 Roscoff, France.
|Source||Ecotoxicology (0963-9292) (Springer), 2009-04 , Vol. 18 , N. 3 , P. 364-376|
|WOS© Times Cited||6|
|Keyword(s)||Microcosm, Phytoplankton community, Epoxiconazole, TTGE, Flow cytometry, HPLC|
|Abstract||The impacts of the fungicide Opus(A (R)) (epoxiconazole) on marine phytoplankton communities were assessed in a 12-day field experiment using in situ microcosms maintained underwater at 6 m depth. Three community analysis methods were compared for their sensitivity threshold in fungicide impact detection. When phytoplankton communities were exposed to 1 mu g l(-1) of epoxiconazole, no effects could be demonstrated using TTGE (Temporal Temperature Gradient gel Electrophoresis), flow cytometry or HPLC. When exposed to 10 mu g l(-1), TTGE fingerprints from PCR amplified 18S rDNA of communities exhibited significant differences compared with controls (ANOSIM, P = 0.028). Neither flow cytometry counts, nor HPLC pigment profiles allowed to show significant differences in microcosms exposed to 10 mu g l(-1) of epoxiconazole. When exposed to 100 mu g l(-1), all three methods allowed to detect significant differences in treated microcosms, as compared to controls. The TTGE analysis appears in this study as the most sensitive method for fungicide impact assessment on eukaryote microbial communities.|