One Perkinsus species may hide another: characterization of Perkinsus species present in clam production areas of France

Type Article
Date 2012-11
Language English
Author(s) Arzul IsabelleORCID1, Chollet Bruno1, Michel J1, Robert Mill Hugh1, Garcia Celine1, Joly Jean-Pierre1, Francois Cyrille1, Miossec Laurence2
Affiliation(s) 1 : IFREMER, Lab Genet & Pathol, F-17390 La Tremblade, France.
2 : IFREMER, Dept Data Dev Integrated Management & Survey, F-44311 Nantes 03, France.
Source Parasitology (0031-1820) (Cambridge Univ Press), 2012-11 , Vol. 139 , N. 13 , P. 1757-1771
DOI 10.1017/S0031182012001047
WOS© Times Cited 24
Keyword(s) clam parasites, Perkinsus chesapeaki, Perkinsus olseni, Ruditapes philippinarum, Ruditapes decussatus, ribosomal RNA, actin, ISH
Abstract Although clam populations in France are known to be infected with protozoans of the genus Perkinsus, no molecular characterization was previously performed on these parasites. Considering that several members of this genus have been associated with mortalities of molluscs worldwide, a study was undertaken in order to characterize these parasites in France. For that purpose, clams, Ruditapes philippinarum and R. decussatus, collected from different production areas and found to be infected with Perkinsus sp. in thioglycolate culture medium, were selected for PCR-RFLP tests and sequencing. Perkinsus olseni was detected in all the investigated areas and results also suggested the presence of P. chesapeaki in Leucate, a lagoon on the Mediterranean coast and in Bonne Anse in Charente Maritime, on the Atlantic coast. Clonal cultures from both detected species were produced in order to describe and compare in vitro stages. Differences in size between both Perkinsus spp. were noticed especially for schizonts and zoosporangia. Lastly, in situ hybridization tests allowed confirmation of the presence of both species in the same R. decussatus population and even in same clams. This is the first detection of P. chesapeaki in Ruditapes species and outside North America, which questions its introduction into Europe.
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Arzul Isabelle, Chollet Bruno, Michel J, Robert Mill Hugh, Garcia Celine, Joly Jean-Pierre, Francois Cyrille, Miossec Laurence (2012). One Perkinsus species may hide another: characterization of Perkinsus species present in clam production areas of France. Parasitology, 139(13), 1757-1771. Publisher's official version : https://doi.org/10.1017/S0031182012001047 , Open Access version : https://archimer.ifremer.fr/doc/00094/20542/