Thiol- and Biotin-Labeled Probes for Oligonucleotide Quartz Crystal Microbalance Biosensors of Microalga Alexandrium Minutum
| Type | Article | ||||||||
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| Date | 2012-07-04 | ||||||||
| Language | English | ||||||||
| Author(s) | Lazerges Mathieu1, 2, Perrot Hubert1, 2, Rabehagasoa Niriniony1, 2, Compere Chantal 3 |
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| Affiliation(s) | 1 : Centre National de la Recherche Scientifique (CNRS), Unité Propre de Recherche (UPR) 15, Laboratoire Interfaces et Systèmes Electrochimiques, 4 place Jussieu, Paris 75252, France 2 : Université Pierre et Marie Curie, LISE, 4 place Jussieu, Paris 75252, France 3 : Institut Français de Recherche pour l’Exploitation de la Mer (IFREMER), Centre de Brest Service Interfaces et Capteurs, Z.I. pointe du diable, Plouzané 29280, France |
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| Source | Biosensors (2079-6374) (MDPI), 2012-07-04 , Vol. 2 , N. 3 , P. 245-254 | ||||||||
| DOI | 10.3390/bios2030245 | ||||||||
| Keyword(s) | Alexandrium Minutum, avidin, biosensor, biotin, DNA, quartz crystal miocrobalance, self-assembled monolayer, thiol | ||||||||
| Abstract | Two quartz crystal microbalance oligonucleotide biosensors of a toxic microalga gene sequence (Alexandrium Minutum) have been designed. Grafting on a gold surface of 20-base thiol- or biotin-labeled probe, and selective hybridization with the complementary 20-base target, have been monitored in situ with a 27 MHz quartz crystal microbalance under controlled hydrodynamic conditions. The frequency of the set up is stable to within a few hertz, corresponding to the nanogram scale, for three hour experiments. DNA recognition by the two biosensors is efficient and selective. Hybridization kinetic curves indicate that the biosensor designed with the thiol-labeled probe is more sensitive, and that the biosensor designed with the biotin-labeled probe has a shorter time response and a higher hybridization efficiency. | ||||||||
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