Comparison of the effects of the dietary addition of two lactic acid bacteria on the development and conformation of sea bass larvae, Dicentrarchus labrax, and the influence on associated microbiota

Type Article
Date 2013-02
Language English
Author(s) Lamari Faouzi1, 2, Castex Mathieu3, Larcher Thibaut4, Ledevin Mireille4, Mazurais DavidORCID1, Bakhrouf Amina2, Gatesoupe Joel5
Affiliation(s) 1 : IFREMER, Ctr Brest, UMR LEMAR 6539, Lab Adaptat Reprod Nutr ARN, F-29280 Plouzane, France.
2 : Fac Pharm, Lab Anal Traitement & Valorisat Polluants Environ, Monastir 5000, Tunisia.
3 : Lallemand Anim Nutr, F-31702 Blagnac, France.
4 : INRA, APEX UMR0703, Oniris, F-44307 Nantes 3, France.
5 : IFREMER, Ctr Brest, Lab Adaptat Reprod Nutr ARN, INRA,UR Nutr Metab Aquaculture 1067, F-29280 Plouzane, France.
Source Aquaculture (0044-8486) (Elsevier Science Bv), 2013-02 , Vol. 376 , P. 137-145
DOI 10.1016/j.aquaculture.2012.11.016
WOS© Times Cited 37
Keyword(s) Fish larvae, Probiotics, Microbiota, Histopathology, Gene expression, DGGE
Abstract Probiotics may have many effects on health and development of fish larvae. One of the most promising is related to spinal conformation, though the mode of action is not clearly understood. The present study attempted to investigate the effects of two strains of lactic acid bacteria on associated microbiota, histological development and gene expression. Sea bass larvae were fed since 5 dph (day post hatch) with either a standard control diet (Diet C), or the
same diet supplemented with Pediococcus acidilactici MA18/5M (Diet P), or with an autochthonous strain of Lactobacillus casei (X2; Diet L). The two lactic acid bacteria were incorporated in the diets at the levels of 106 and 107 CFU (colony forming units) g-1 in two consecutive experiments, respectively. The experimental treatments maintained the lactic acid bacteria above the detection threshold in the larvae. In the second experiment, the Bray-Curtis indices revealed the dissimilarity between the Bacterial Community Profiles (BCPs) associated with Diet P and those of the two other dietary groups. The two lactic acid bacteria promoted growth, especially by 20-22 dph, but the development seemed affected differently in the two groups. The osteocalcin gene was overexpressed at 20-22 dph in group L, suggesting a difference in the early bone development compared with Group P. A possible consequence was the highest incidence of spinal deformities in Group L. At day 62 dph, the ossification was achieved and normal in 60% of the larvae in Group P, whereas this rate was only 13 and 19% in Groups C and L, respectively. The evaluation of probiotics should not be therefore limited to growth measurements, and should take into account ontogenetic chronology for improving larval quality with such treatments.
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Lamari Faouzi, Castex Mathieu, Larcher Thibaut, Ledevin Mireille, Mazurais David, Bakhrouf Amina, Gatesoupe Joel (2013). Comparison of the effects of the dietary addition of two lactic acid bacteria on the development and conformation of sea bass larvae, Dicentrarchus labrax, and the influence on associated microbiota. Aquaculture, 376, 137-145. Publisher's official version : https://doi.org/10.1016/j.aquaculture.2012.11.016 , Open Access version : https://archimer.ifremer.fr/doc/00108/21966/