Improvement of chilled seabass sperm conservation using a cell culture medium

Type Article
Date 2012-12
Language English
Author(s) Fauvel Christian1, Boryshpolets Sergey2, Cosson J.2, Leedy J. G. Wilson3, Labbe C.4, Haffray Pierrick5, Suquet Marc6
Affiliation(s) 1 : IFREMER, CNRS, IRD, Stn Expt Aquaculture,UM1,UM2,UMR ECOSYM 5119, F-34250 Palavas Les Flots, France.
2 : Univ S Bohemia Ceske Budejovice, Fac Fisheries & Protect Waters, S Bohemian Res Ctr Aquaculture & Biodivers Hydroc, Vodnany, Czech Republic.
3 : Univ Idaho, Dept Biol Sci, Ctr Reprod Biol, Moscow, ID 83843 USA.
4 : INRA, UR Scribe 1037, F-35042 Rennes, France.
5 : SYSAAF, Scribe, Rennes, France.
6 : IFREMER, Stn Expt Argenton, UMR 100, Argenton, France.
Meeting Proceedings of the Third International Workshop on the “Biology of Fish Gametes” : Budapest, Hungary, 7–9 September 2011
Source Journal Of Applied Ichthyology (0175-8659) (Wiley-blackwell), 2012-12 , Vol. 28 , N. 6 , P. 961-966
DOI 10.1111/jai.12071
WOS© Times Cited 13
Abstract The sperm of seabass is very fragile and it quickly loses its ability to fertilize after collection either if kept undiluted or in classic saline media. In order to avoid cryopreservation when only short conservation is required, the process of sperm management including sperm collection, sperm dilution rate in storage medium and storage medium composition, was subject to experimental trials. A concentration of 20% urine generated a low pH of seminal fluid, and it immediately altered the motility ability. However, pH did not seem to be the key agent of motility prevention since sperm dilution in Leibovitz culture medium (L15) or in classic saline medium both presenting a similar low pH (7.3) did not affect motility. L15 increased the duration of sperm survival by 2 days at 4 degrees C after collection. Moreover, dilution could be restricted to 1 : 3 (v : v) for conservation of chilled sperm. Chilled sperm could be cryopreserved with no more damages than those observed after freezing of fresh sperm.
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Fauvel Christian, Boryshpolets Sergey, Cosson J., Leedy J. G. Wilson, Labbe C., Haffray Pierrick, Suquet Marc (2012). Improvement of chilled seabass sperm conservation using a cell culture medium. Journal Of Applied Ichthyology, 28(6), 961-966. Publisher's official version : , Open Access version :