Study of the antioxidant capacity in gills of the Pacific oyster Crassostrea gigas in link with its reproductive investment

Type Article
Date 2013-01
Language English
Author(s) Beguel Jean-Philippe1, Huvet ArnaudORCID2, Quillien Virgile2, Lambert Christophe1, Fabioux Caroline1
Affiliation(s) 1 : Univ Bretagne Occidentale, Inst Univ Europeen Mer, Lab Sci Environm Marin, CNRS UBO IRD IFREMER,UMR 6539, F-29280 Plouzane, France.
2 : CNRS UBO IRD IFREMER, UMR 6539, Lab Physiol Invertebres, IFREMER, F-29280 Plouzane, France.
Source Comparative Biochemistry And Physiology C-toxicology & Pharmacology (1532-0456) (Elsevier Science Inc), 2013-01 , Vol. 157 , N. 1 , P. 63-71
DOI 10.1016/j.cbpc.2012.10.004
WOS© Times Cited 18
Keyword(s) Antioxidant capacity, Crassostrea gigas, Reproduction, RNA interference, ROS
Abstract Energy allocation principle is a core element of life-history theory in which "the cost of reproduction" corresponds to an acceleration of senescence caused by an increase in reproductive investment. In the "theory of aging", senescence is mainly due to the degradation of lipids, proteins and DNA by reactive oxygen species (ROS), by-products of oxidative metabolism. Some studies have shown that oxidative stress susceptibility could be a cost of reproduction. The present study investigates the effect of reproductive investment on antioxidant capacity in the gills of a species with a very high reproductive investment, the Pacific oyster Crassostrea gigas. We used RNA interference targeting the oyster vasa-like gene (Oyvlg) to produce oysters with contrasted reproductive investment. Antioxidant capacity was studied by measuring the mRNA levels of genes encoding major antioxidant enzymes, and the activity of these enzymes. The highest reproductive investment was associated with the highest transcript levels for glutathione peroxidase and extra-cellular and mitochondrial superoxide dismutase. In contrast, lipid peroxidation did not show any sign of oxidative damage whatever the reproductive investment. Up-regulation of certain genes encoding enzymes involved in the first step of ROS detoxification could therefore be a part of the organism's strategy for managing the pro-oxidant species produced by heavy reproductive investment. (C) 2012 Elsevier Inc. All rights reserved.
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Beguel Jean-Philippe, Huvet Arnaud, Quillien Virgile, Lambert Christophe, Fabioux Caroline (2013). Study of the antioxidant capacity in gills of the Pacific oyster Crassostrea gigas in link with its reproductive investment. Comparative Biochemistry And Physiology C-toxicology & Pharmacology, 157(1), 63-71. Publisher's official version : https://doi.org/10.1016/j.cbpc.2012.10.004 , Open Access version : https://archimer.ifremer.fr/doc/00116/22744/