Identifying the viral genes encoding envelope glycoproteins for differentiation of Cyprinid herpesvirus 3 isolates
|Author(s)||Han 1, 2, Kim Ji Hyung3, Renault Tristan4, Choresca Casiano, Jr.1, 2, Shin Sang Phil1, 2, Jun Jin Woo1, 2, Park Se Chang1, 2|
|Affiliation(s)||1 : Seoul Natl Univ, Coll Vet Med, Lab Aquat Biomed, Seoul 151742, South Korea.
2 : Seoul Natl Univ, Res Inst Vet Sci, Seoul 151742, South Korea.
3 : Korea Ocean Res & Dev Inst, Ansan 426744, South Korea.
4 : IFREMER, LGP, F-17390 La Tremblade, France.
|Source||Viruses-basel (1999-4915) (Mdpi Ag), 2013-02 , Vol. 5 , N. 2 , P. 568-576|
|WOS© Times Cited||8|
|Keyword(s)||Cyprinid herpes virus 3, glycoprotein gene, Koi herpesvirus, microsatellite, polymorphism, phylogenetic tree|
|Abstract||Cyprinid herpes virus 3 (CyHV-3) diseases have been reported around the world and are associated with high mortalities of koi (Cyprinus carpio). Although little work has been conducted on the molecular analysis of this virus, glycoprotein genes identified in the present study seem to be valuable targets for genetic comparison of this virus. Three envelope glycoprotein genes (ORF25, 65 and 116) of the CyHV-3 isolates from the USA, Israel, Japan and Korea were compared, and interestingly, sequence insertions or deletions were observed in these target regions. In addition, polymorphisms were presented in microsatellite zones from two glycoprotein genes (ORF65 and 116). In phylogenetic tree analysis, the Korean isolate was remarkably distinguished from USA, Israel, Japan isolates. These findings may be suitable for many applications including isolates differentiation and phylogeny studies|