Cryopreservation of gametophytes of Laminaria digitata (L) lamouroux by encapsulation dehydration

Mot-clé(s)

alga, Laminaria digitata, gametophyte, cryopreservation, encapsulation, dehydration.

A cryopreservation procedure was developed for gametophytes of Laminaria digitata using an encapsulation-dehydration technique. Cells were encapsulated in calcium alginate beads pretreated for 6 h in liquid medium containing 0.3 to 0.5 M sucrose and then dehydrated. Freezing was performed in two steps, the first consisting of a slow temperature decrease from 19°C to -40°C and the second of direct immersion of samples in liquid nitrogen. Thawing was performed in a water bath at 40°C for 2 min. Survival rates were between 25 and 75% depending on age, sex and stress. The best results were obtained with alginate beads pretreated for 6 h in a 0.3-0.4 M sucrose solution and dehydrated down to 40% of the initial wet weight. Recovery time was about two weeks.

Full Text

FilePagesSizeAccess
Author's final draft
6205 Ko
How to cite
Vigneron Thierry, Arbault Suzanne, Kaas Raymond (1997). Cryopreservation of gametophytes of Laminaria digitata (L) lamouroux by encapsulation dehydration. Cryo-letters. 18 (2). 93-98. https://archimer.ifremer.fr/doc/00125/23643/

Copy this text