Cryopreservation of gametophytes of Laminaria digitata (L) lamouroux by encapsulation dehydration

Type Article
Date 1997-03
Language English
Author(s) Vigneron Thierry, Arbault Suzanne1, Kaas Raymond1
Affiliation(s) 1 : Ifremer, France
Source Cryo-letters (0143-2044), 1997-03 , Vol. 18 , N. 2 , P. 93-98
WOS© Times Cited 20
Mot-Clé(s) alga, Laminaria digitata, gametophyte, cryopreservation, encapsulation, dehydration.
Abstract A cryopreservation procedure was developed for gametophytes of Laminaria digitata using an encapsulation-dehydration technique. Cells were encapsulated in calcium alginate beads pretreated for 6 h in liquid medium containing 0.3 to 0.5 M sucrose and then dehydrated. Freezing was performed in two steps, the first consisting of a slow temperature decrease from 19°C to -40°C and the second of direct immersion of samples in liquid nitrogen. Thawing was performed in a water bath at 40°C for 2 min. Survival rates were between 25 and 75% depending on age, sex and stress. The best results were obtained with alginate beads pretreated for 6 h in a 0.3-0.4 M sucrose solution and dehydrated down to 40% of the initial wet weight. Recovery time was about two weeks.
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Vigneron Thierry, Arbault Suzanne, Kaas Raymond (1997). Cryopreservation of gametophytes of Laminaria digitata (L) lamouroux by encapsulation dehydration. Cryo-letters, 18(2), 93-98. Open Access version : https://archimer.ifremer.fr/doc/00125/23643/