Low-energy and low-cost separation of microalgae from water is important to the economics of microalgae harvesting and processing. Flotation under vacuum using a vacuum gas lift for microalgae harvesting was investigated for different airflow rates, bubble sizes, salinities and harvest volumes. Harvesting efficiency (HE) and concentration factor (CF) of the vacuum gas lift increased by around 50% when the airflow rate was reduced from 20 to 10 L min(-1). Reduced bubble size multiplied HE and CF 10 times when specific microbubble diffusers were used or when the salinity of the water was increased from 0 parts per thousand to 40 parts per thousand. The reduction in harvest volume from 100 to 1 L increased the CF from 10 to 130. An optimized vacuum gas lift could allow partial microalgae harvesting using less than 0.2 kWh kg(-1) DW, thus reducing energy costs 10-100 times compared to complete harvesting processes, albeit at the expense of a less concentrated biomass harvest. (C) 2012 Elsevier Ltd. All rights reserved.